Genistein对高钾和化学缺氧所致人视网膜色素上皮细胞损伤的保护作用  

Effects of genistein on the injury induced by KCl and CoCl2 in cultured human retinal pigment epithelium cells

作　　者：邹颖[1] 李华[1] 严虹[1] 潘金顺[1] 王斌[1] 

机构地区：[1]南京医科大学药理学系

出　　处：《南京医科大学学报（自然科学版）》2005年第1期5-8,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学青年基金资助项目(30000207);江苏省教育厅重点资助项目(KJB310001);江苏省高校高新技术产业化资助项目(JH01-050);南京医科大学创新基金资助项目(CX2001003);校基金资助项目(NY03027)

摘　　要：目的:研究Genistein(gen)对高钾和化学缺氧所致人视网膜色素上皮(retinalpigmentepithelium,RPE)细胞损伤的保护作用。方法:采用体外培养人RPE细胞,MTT法测定细胞存活率以及在倒置相差显微镜下观察细胞形态改变。结果:200mmol/LKCl作用12h可降低细胞存活率至(43.97±3.43)%,gen50、100、200μmol/L可明显提高细胞存活率且呈浓度依赖性。相差显微镜观察细胞形态发现,200mmol/LKCl作用2h细胞膜开始皱缩,4h胞膜皱缩更加明显,胞核也开始浓缩,8h后仅见细胞核和断裂呈絮状的细胞膜,12h仅可见固缩的细胞核,而200μmol/Lgen可以减轻细胞损伤,4h后方见细胞膜开始皱缩;CoCl2损伤模型中,500μmol/LCoCl2作用12h可降低细胞存活率至(57.81±17.19)%,gen50、100、200μmol/L时也可浓度依赖性升高细胞存活率。结论:Gen对高钾和化学缺氧所致人RPE细胞损伤具有保护作用。Objective: To investigate the protective effects of genistein(gen) on human retinal pigment epithelium(RPE) cells injury induced by KCl and CoCl2. Methods: Injury was induced by KCl and CoCl2 in RPE cells. The cells viability was measured by MTT assay and morphological changes of the cells were recorded. Results:KCl could significantly decrease the survival rate of RPE cells at 12 h, and gen markedly increased the survival rate in a concentration-dependent manner. RPE cells presented progressively destruction of cell membrane at 2 h and pycnosis of cell nuclear at 4 h after 200 mmol/L KCl treatment. At 8 h after KCl treatment, pycnosis and batt-liked cell membrane could be seen. Only pycnosis could be observed at 12 h. These changes could be inhibited by 200 μmol/L gen. CoCl2 could also inhibit the survival rate of RPE cells and gen could concentration-dependently elevate the survival rate. No significant morphological changes of RPE cells induced by CoCl2 were found. Conclusion: Gen could be effective agent in protection on the injury induced by KCl and CoCl2 in cultured RPE cells.

关 键 词：GENISTEIN KCl CoCl2 人视网膜色素上皮细胞 损伤 保护 

分 类 号：R774.13[医药卫生—眼科]