Effects of dendritic cells transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response  

作　　者：Hua-WenSun Qi-BingTang Yong-JunCheng Sheng-QianZou 

机构地区：[1]DepartmentofGeneralSurgery,RenminHospital,WuhanUniversity,Wuhan430060,HubeiProvince,China [2]DepartmentofGeneralSurgery,TongjiHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430030,HubeiProvince,China

出　　处：《World Journal of Gastroenterology》2004年第17期2595-2597,共3页世界胃肠病学杂志（英文版）

摘　　要：AIM: To investigate the effects of dendritic cells (DCs) transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response.METHODS: The wild-type p53 was transduced to DCs with adenovirus, and the DCs were stimulated by gastric cancer lysates. The surface molecules (B7-1, B7-2, MHC-I, MHC-II) of all DCs were detected by FACS, and the ability of the DCs to induce efficient and specific immunological response inanti-^51Cr-labeled target cells was studied. BALB/c mice injected with DCs and Mk28 were established, and CTL response in mice immunized with Lywt-p53DC was evaluated.Tumor-bearing mice were treated with Lywt-p53DC.RESULTS: The surface molecules of Lywt-p53DC had a high expression of B7-1 (86.70±0.07%), B7-2 (18.77±0.08%),MHC-I (87.20±0.05%) and MHC-II (56.70±0.07%); T lymphocytes had a specific CTL lysis ability induced by Lywt-p53DC; the CTL lysis rate was as high as 81%. The immune protection of Lywtp-53DC was obvious, the tumor diameter in Lywtp-53DC group was 3.10+0.31 ram, 2.73±0.23 ram,3.70±0.07 mm on d 13, 16 and 19, respectively, which were smaller than control, DC, wtp53DC and LyDC group (P<0.05). Tumor growth rate in Lywtp53DC group was slower than that in other groups (P<0.05).CONCLUSION: DCs transfected with wild-type p53 and stimulated by gastric cancer lysates have specific CTL killing activity.AIM:To investigate the effects of dendritic cells(DCs) transfected with full-length wild-type p53 and stimulated by gastric cancer lysates on immune response. METHODS:The wild-type p53was transduced to DCs with adenovirus,and the DCs were stimulated by gastric cancer lysates.The surface molecules(B7-1,B7-2,MHC-Ⅰ,MHC-Ⅱ) of all DCs were detected by FACS,and the ability of the DCs to induce efficient and specific immunological response in anti-^(51)Cr-labeled target cells was studied.BALB/c mice injected with DCs and Mk28 were established,and CTL response in mice immunized with Lywt-p53DC was evaluated. Tumor-bearing mice were treated with Lywt-p53DC. RESULTS:The surface molecules of Lywt-p53DC had a high expression of B7-1(86.70±0.07%),B7-2(18.77±0.08%), MHC-Ⅰ(87.20±0.05%)and MHC-Ⅱ(56.70±0.07%);T lymphocytes had a specific CTL lysis ability induced by Lywt- p53DC;the CTL lysis rate was as high as 81%.The immune protection of Lywtp-53DC was obvious,the tumor diameter in Lywtp-53DC group was 3.10±0.31 mm,2.73±0.23mm, 3.70±0.07 mm on d 13,16 and 19,respectively,which were smaller than control,DC,wtp53DC and LyDC group (P<0.05).Tumor growth rate in Lywtp53DC group was slower than that in other groups(P<0.05). CONCLUSION:DCs transfected with wild-type p53 and stimulated by gastric cancer lysates have specific CTL killing activity.

关 键 词：树枝状细胞 基因转染 全长性 野生性 P53 刺激作用 胃癌 肿瘤 溶解产物 免疫反应 消化系统 

分 类 号：R735.2[医药卫生—肿瘤]