久仰香猪染色体C-带多态性研究  

The Chromosome C-bands of Jiuyang Xiang-pig

作　　者：张依裕[1] 刘培琼[1] 徐如宏[2] 林家栋[3] 张明忠 

机构地区：[1]贵州大学香猪研究所,贵阳550025 [2]贵州大学麦作研究中心,贵阳550025 [3]贵州大学动物科学系,贵阳550025 [4]贵州省品改站,贵阳550002

出　　处：《贵州农业科学》2004年第6期7-10,共4页Guizhou Agricultural Sciences

基　　金：国家重点基础研究规划"973"项目 (G2 0 0 0 0 1 61 0 4 )

摘　　要：以久仰香猪为试验组 ,剑白香猪、从江香猪和长白猪为对照组 ,用外周血淋巴细胞培养 ,C 分带处理。结果表明 :各染色体均不同程度显带 ,发生在着丝粒、次缢痕及其附近和长臂上 ,呈圆形或椭圆形且存在多态性 ;染色体C 带在细胞周期和猪的不同生长时期具有相对稳定性 ;染色体C 带大小与染色体长度无关 ;在个体内细胞间同一染色体C 带大小具有高度可重复性 ;大型带发生在Y 染色体、1、2、13～ 16、18号染色体上 ,Y 染色体C带几乎占据整个长臂 ,呈椭圆形 ;中型带发生在 3～ 8、12、17号染色体上 ;小型带发生在 9、10、11、X染色体上 ;香猪类型之间显带频率、相对长度和面积均较高 ,长白猪相对较低 ,说明香猪异染色质含量比长白猪高。Jiuyang Xiang-pig was chosen as the test group. Jianbai Xiang-pig, Congjiang Xiang-pig and Landrace as ckeck groups. The C-bands of their chromosomes were studied by the method of peripheral blood lymphocytes culture and C-banding. The results showed that all of chromosomes appeared C-bands in different degrees and had polymorphism,they were located in centromere or its neighborhood or long arm, and presented circularity or ellipse. The chromosome C-bands had relative stability in cell periods and different growth stages of pigs, its size and chromosome lengths had not relevance. The C-band had high repeat in the same chromosome of individual between cells. The C-bands of No.1, 2, 13 to 16,18 and Y chromosome appeared large range, the (Y-chromosome) has a elliptic C-band, which nearly (occupied) the whole long arm.The midium-sized C-banding were found on (chromosomes) No.3 to 8,12 and 17. Other chromosomes (9,10,11 and X) appeared small range. The C-banding area, relative length and occurred frequency of chromosomes of Xiang-pig were more higher than Landrace, indicated that Xiang-pig had more (heterochromatin) than Landrace.

关 键 词：白香猪 长白猪 17号染色体 发生 生长时期 C-带 多态性研究 18号染色体 对照组 体内 

分 类 号：S828[农业科学—畜牧学] R735[农业科学—畜牧兽医]