环糊精糖基转移酶(CGTase)的化学修饰  

Study on Effects of Protein Modification Reagents on CGTase

作　　者：曹新志[1] 金征宇[1] 

机构地区：[1]江南大学食品学院,江苏无锡214036

出　　处：《食品科学》2004年第12期64-68,共5页Food Science

摘　　要：不同蛋白质修饰剂对环糊精葡萄糖基转移酶进行修饰。在一定条件下,分别用丁二酮(DIC)、苯甲酰磺酰氟(PMSF)、二硫苏糖醇(DTT)和氯氨-T(Ch-T)处理后,酶活不受影响,说明精氨酸残基、羟基、二硫键和蛋氨酸残基与酶的活力无关。用焦碳酸二乙酯(DEPC)、N-溴代琥珀酰亚胺(NBS)和碳化二亚胺(EDC)修饰后,酶活力大幅度下降,说明组氨酸、色氨酸和羧基氨基酸为酶活力所必需。The effects of some protein modification reagents on the activity of cyclodextrin glucanotransferase(CGTase) have been studied. That the enzyme was not affected by DIC, PMSF, DTT and Ch-T modification, suggested that guanido, hydroxyl, disulfide bond and mehionine residues were insignificant to enzyme activity. The enzyme activity was remarkably decreased after DEPC, NBS, EDC modification.The results indicated that carboxyl group, imidazolyl group and indolyl group seemed to be significant to the catalytic activity of cyclodextrin glucantransferase.

关 键 词：嗜碱芽孢杆菌 环糊精葡萄糖基转移酶 化学修饰 

分 类 号：Q556[生物学—生物化学]