CIK细胞对MGC-803胃癌细胞株的诱导凋亡作用  

Inducing apoptosis effects and mechanism investigation of CIK cells against MGC-803 gastric cancer cell lines

作　　者：李雪梅[1] 孙抒[2] 王建光[1] 杨万山[2] 

机构地区：[1]温州医学院附属二院,温州325027 [2]延边大学医学院病理教研室,延边133000

出　　处：《实用肿瘤学杂志》2004年第6期413-416,共4页Practical Oncology Journal

基　　金：吉林省科学技术厅资助课题 (No :2 0 0 10 5 5 7)

摘　　要：目的　研究多种细胞因子诱导的杀伤细胞(CYtokine-inducedkillcells,CIK)对MGC -80 3胃癌细胞株的诱导凋亡作用,并探讨其作用机制。方法　应用(TdT -mediateddUTPnickendla beling)TUNEL法检测CIK细胞诱导凋亡的情况;通过免疫细胞化学法测定Bcl- 2、Bax的阳性表达率,深入研究CIK细胞对MGC - 80 3胃癌细胞株凋亡相关基因蛋白表达的影响。结果　TUNEL法检测对照组成不染或均匀淡蓝色,实验组凋亡细胞缩小,核或核周成深蓝色。CIK实验组5～1 4小时凋亡率上调,1 4～2 4小时凋亡率下降。CIK实验组凋亡率与对照组相比有显著性差异(P <0 .0 1 )。免疫细胞化学结果表明:Bcl- 2蛋白随作用时间延长表达均下降,Bax蛋白表达上调,与对照组相比均有显著性差异(P <0 .0 1 )。结论　CIK细胞对MGC - 80 3胃癌细胞早期诱导凋亡,晚期诱导肿瘤细胞坏死。其作用机制之一可能是通过上调Bax,下调Bcl- 2的蛋白表达实现的。Objective To study the anti-proliferation and inducing apoptosis effects of cytokine-induced killer cells-CIK cells for MGC-803 gastric cancer celL lines and to probe its under1ying mechanism. Methods Inducing apoptosis of CIK cells on MGC-803 gastric cancer cell line was evaluated by TUNEL methods; The positive expression of Bcl-2 and Bax were determined by immunocytochemistry (ICC). The expression changes of relating-apoptosis gene proteins were deeply studied.Results TUNEL showed that the cells of control group were not to stained or stained average light blue and the cells of experiment group became small and nucleus were dyed deeply blue. The apoptosis rate of CIK experiment group increased in 5～14 hours and declined in 14～24 hours. The apoptotic rate of CIK experiment groups have significant difference compared with control (P< 0.01); Immunocytochemistry showed that expression of p53?pl6?C-myc?Bcl-2 protein in CIK experiment group inclined and Bax protein increased when time was continued and there were significant difference compared with control (P< 0.01).Conclusion CIK cells induce apoptosis of MGC-803 gastric cancer cell line in early phase and induce necrocytosis in late phase. One of the mechanisms is that it can inhance Bax proteins expression and decline Bcl-2 proteins expression of tumor cells.

关 键 词：MGC-803 CIK细胞 诱导凋亡 胃癌细胞株 凋亡率 对照组 表达 免疫细胞化学 凋亡细胞 蓝色 

分 类 号：R735.2[医药卫生—肿瘤]