极大螺旋藻胞内多糖分离纯化及其结构的初步分析  

ISOLATION AND PURIFICATION OF INTRACELLULAR POLYSACCHARIDE FROM SPIRULINA MAXIMA AND ITS PRIMARY STRUCTURE

作　　者：张威[1] 朱劲华[1] 王敏[2] 李朝军[2] 张成武[2] 

机构地区：[1]江苏职工医科大学,南京210029 [2]南京师范大学江苏省分子医学生物技术重点实验室,南京210097

出　　处：《天然产物研究与开发》2004年第6期548-551,共4页Natural Product Research and Development

摘　　要：本文以原产于非洲乍得湖的极大螺旋藻 (Spirulinamaxima)为材料 ,热水提取胞内粗多糖 ,丙酮、乙醚脱水脱色 ,再经改良的Sevag等方法脱蛋白 ,获得胞内多糖半精品 (Intracellularpolysaccharide ,IPSⅠ ) ,经DE 5 2、SephadexG 10 0层析柱纯化 ,得到两种单一组分的多糖精品 :IPSⅡA和IPSⅡB。利用HPLC、IR等方法测定其初步结构。结果显示 :IPSⅡA的平均分子量为 2 38,0 0 0Dt,主要单糖组成为L 岩藻糖、L 鼠李糖、D 木糖、D 半乳糖 (摩尔比为 6 .0 9∶3.38∶0 .6 6∶0 .0 72 ) ,硫酸根含量为 2 6 .3± 3.7μg/mg ,糖醛酸含量约为 13.37± 0 .4 5 μg/mg ;IPSⅡB的平均分子量为 34,90 0Dt ,主要单糖组成包括D 木糖、L 鼠李糖、D 果糖、D 葡萄糖、L 岩藻糖、D 甘露糖 (摩尔比为 6 .6 6∶4 .6 3∶0 .2 8∶0 .12∶0 .0 87∶0 .0 2 2 ) ,糖苷键构型以α型为主 ,硫酸根含量为2 1.5± 1.2 μg/mg。We obtained the semi-rarefied intracellular polysaccharide (IPSⅠ) from Spirulina maxima,originating from Chad lake in Africa,by hot water,acetone,aether and improved Sevag's method. IPSⅠwas further purified by loading on DE-52 and Sephadex G-100,respectively.Two pure fractions (IPSⅡA and IPSⅡB) were obtained by above homogenous,and their primary structures and biology functions are investigated by HPLC,IR. Our results showed that: IPSⅡA with 238,000 Dt molecular weight,was mainly composed of L-fucose,L-rhamnose,D-xylose and D-mannose (the molar ratio,6.09∶3.38∶0.66∶0.072),uronic acids(13.37±0.45 μg/mg) and sulfate(26.3±3.7 μg/mg); IPSⅡB with 34,900 Dt molecular weight,was consisted of D-xylose,L-rhamnose,D-fructose,D-glucose,L-focose and D-galactose (the molar ratio,6.66∶4.63∶0 28∶0.12∶0.087∶0.022) and sulfate (21.5±1.2 μg/mg),and mainly linked by α-glycosidics in its backbone.

关 键 词：单糖组成 含量 D-半乳糖 HPLC 鼠李糖 粗多糖 蛋白 摩尔比 胞内多糖 木糖 

分 类 号：Q53[生物学—生物化学] R285.5[医药卫生—中药学]