应用近红外透射光谱法测定水稻种子直链淀粉含量的初步研究  

Application of Near Infrared Transmittance Spectroscopy (NITS) to the Determination of Amylose Content of Rice Grain

作　　者：肖昕[1] 谢新华[1] 陈奕[1] 罗文永[1] 陈建伟[1] 刘彦卓[1] 毛兴学[1] 李晓方[1] 

机构地区：[1]广东省农业科学院水稻研究所农业部水稻遗传改良重点开放实验室/广东省水稻育种新技术重点实验室,广州510640

出　　处：《中国农业科学》2004年第11期1709-1712,共4页Scientia Agricultura Sinica

基　　金：国家自然科学基金重大项目(39893350);广东省自然科学基金项目(020384);农业部"948"项目资助

摘　　要：以124份水稻种子为样本,利用FOSS-Tecator公司的Infratec1255型近红外谷物品质分析仪,对样本进行光谱扫描,并利用化学法测定了直链淀粉含量。借助于近红外定标软件(WinISI),采用多种计量数学处理方法和不同的回归统计方法进行定标曲线的开发和比较,得到了小样本量水稻种子直链淀粉含量测定的近红外分析定标数学模型。其定标标准偏差(SEC)、交叉检验标准误差(SECV)和定标决定系数(RSQ)分别为1.489、1.761和0.885。One hundred and twenty-four samples of small amount of rice grain were scanned by Foss Tecator Infratec 1255, and their amylose contents were determined by chemistry method. With the WINISI calibration maker software, several mathematics treatments to the sample spectra and several different regression methods were adopted to optimize a good calibration equation for determination of amylose content in small amount of rice grain. The standard error of calibration (SEC), standard error of cross-validation (SECV) and regression squared (RSQ) of the calibration selected were 1.489, 1.761 and 0.885.

关 键 词：近红外透射光谱法 水稻 种子 直链淀粉 含量测定 蒸煮食味品质 

分 类 号：S511[农业科学—作物学]