Simultaneous Determination of Ceftazidime and Tazobactam in Injectable Powder by Reversed-Phase High Performance Liquid Chromatography  

RP-HPLC法同时测定注射用粉末中他唑巴坦和头孢他啶组分的含量(英文)

作　　者：孟湘明[1] 孟志云[1] 张亮[1] 窦桂芳[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850

出　　处：《Journal of Chinese Pharmaceutical Sciences》2004年第4期267-270,共4页中国药学（英文版）

摘　　要：A reversed-phase high performance liquid chromatographic (RP-HPLC) method wasdeveloped and validated for the simultaneous deteimination of ceftazidime and tazobactam ininject-able powder. Methods Chromatography was carried out on Zorbax 300SB-C_(18) column using amixture of methanol and aqueous solution of phosphate buffer (pH = 5.6) as mobile phase. The UVdetection wavelength was 220 run. Results The linear ranges of ceftazidime and tazobactam were 0.62- 631.8 μg·mL^(-1) and 0.66 - 677.50 μg·mL^(-1), respectively. The average recoveries were 98.8%- 101.4% for ceftazidime, and 99,1% - 100.2% for tazobactam. The RSD values of inter-day andintra-day assays were lower than 1.5% for ceftazidime and 2.6% for tazobactam. Conclusion Thismethod is reproducible, simple, precise, and rapid for the quality control of ceftazidime andtazobactam in injectable powder.目的　建立反相高效液相色谱法用于同时测定他唑巴坦和头孢他啶的含量。方法　采用Zorbax 30 0SB C1 8色谱柱 ,流动相为甲醇 磷酸盐缓冲液 (pH =5 6 ) ,检测波长为 2 2 0nm。结果　他唑巴坦和头孢他啶分别在 0 6 2 - 6 31 8μg·mL- 1 和0 6 6 - 6 77 5 μg·mL- 1 内呈线性关系 ,平均加样回收率分别为 98 8%- 1 0 1 4 %和 99 1 %- 1 0 0 2 %,日内和日间精密度分别为0 2 %- 1 5 %和 0 1 %- 2 6 %。结论　该法简单、精确、快速、重复性好 ,可以满足其原料和制剂的质量控制要求。

关 键 词：CEFTAZIDIME TAZOBACTAM RP-HPLC 

分 类 号：R927[医药卫生—药学]