大肠杆菌不耐热肠毒素B亚单位基因工程菌发酵工艺的研究  

Fermentation technology in heat-labile enterotoxin B subunit of genetic recombinant E. coli

作　　者：田文标[1] 邹全明[1] 张卫军[1] 

机构地区：[1]第三军医大学医学检验系临床微生物与免疫学教研室,重庆400038

出　　处：《第三军医大学学报》2005年第2期115-118,共4页Journal of Third Military Medical University

基　　金：国家"九五"重点科技攻关项目 ( 96 90 1 0 1 54 )~~

摘　　要：目的　研究建立大肠杆菌不耐热肠毒素B亚单位 (LTB)基因工程菌的发酵工艺。方法　先通过摇瓶培养初步确定工程菌的最适生长条件 ,然后以此为基础在发酵罐中比较、筛选影响菌体生长和目的蛋白表达率的各种条件 ,并进一步对发酵工艺进行改良优化。结果　经试验确定了rLTB基因工程菌发酵罐培养的各项条件的最佳组合 ,优化后工程菌菌体产量平均可达 40 5g/L ,目的蛋白的表达率平均为 3 0 6%。结论　建立了稳定、高效的工程菌发酵工艺。Objective To investigate the fermentation conditions for the heat-labile enterotoxin B subunit (LTB) of genetic recombinant E.coli. Methods The effects of different kinds of media, the range of pH, the induction time, the glucose concentration, and the fed-batch on LTB level in 10-liter fermenter under the condition of cascade controlling dissolved oxygen content were analyzed. Results Under the established conditions, the cell output per liter was 40.5g, and the expression level was 30.6%. Conclusion The results indicate that the stable fermentation technology with high efficiency has been established.

关 键 词：产毒素性大肠杆菌 不耐热肠毒素B亚单位 发酵 

分 类 号：R378.21[医药卫生—病原生物学] TQ460.38[医药卫生—基础医学]