部分背根切断对备用背根节和脊髓NT-4表达的影响  

The Changes of NT-4 Expression in Spared Root Ganglion and Spinal Cord Following Partial Dorsal Root Rhizotomy

作　　者：龙双涟[1] 刘芬[1] 王廷华[1] 徐新运[1] 关宇光[1] 王特为[1] 柯青[1] 袁源[1] 

机构地区：[1]昆明医学院神经科学研究所,昆明650031

出　　处：《四川大学学报（医学版）》2005年第1期35-38,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金 (批准号 3 0 0 0 0 2 2 7);云南省教委基金( 0 0 12 0 5 1)资助

摘　　要：目的　探讨 NT- 4在正常猫和部分去背根猫的 L6 背根节 (手术侧和非手术侧 )和脊髓 板层与背核表达的变化。方法　成年健康雄猫 15只分为正常组、备用根术后 7d组和 14 d组 (动物行单侧部分去背根手术 ,即切除一侧 L1 - L5、L7- S2 背根节 ,保留 L6 为备用根 ) ,采用免疫组化 ABC法 ,观察、计数各组背根节、脊髓实验侧 板层 (L3、L5、L6 )和背核 (L3)中 NT- 4的分布及含量。结果　 1去部分背根后 7d,备用背根节 (术侧 )中 NT- 4阳性神经元数比正常组显著减少 (P<0 .0 5 ) ,但较非术侧明显增多 (P<0 .0 5 ) ;至术后 14 d,术侧备用背根节 NT- 4阳性神经元比正常组、7d组和非术侧均减少 (P<0 .0 5 )。 2正常组、术后两时相 L3、L5、L6 术侧脊髓 板层和 L3背核内NT- 4阳性神经元数均无明显变化。结论　去部分背根主要导致备用背根节 NT-Objective This study was conducted to detect the expression levels of NT-4 in the spared dorsal root ganglia (DRG,L 6) on the operated side and un-operated side, in the spinal lamina Ⅱ(L 3, L 5, L 6) and Clarke's nucleus (L 3) of the normal adult cats and the cats submitted to partial dorsal rhizotomy. The aim was to illuminate the temporospatial changes of NT-4 expression and its possible relation to spinal cord plasticity. Methods Fifteen male adult cats were divided into three groups. The cats of two groups were submitted to unilateral partial dorsal root rhizotomy (L 1-L 5, L 7-S 2 DRG were sectioned, but L 6 was spared) and animals were sacrificed at survived 7 days and 14 days after operation. The sections were stained by the immunohistochemistry ABC method under the same condition, using NT-4 (1∶200, Santa Cruz) antibody. The distribution and the number of NT-4 positive neurons in bilateral spared DRG (L 6) in the operated/un-operated side, spinal lamina Ⅱ(L 3, L 5, L 6) and Clarke's nucleus (L 3) of each animal were observed and counted. All data were analyzed by one-way ANOVA, SNK-q test and paired-t test. Results (1) Seven days after partial dorsal root rhizotomy, the number of NT-4 positive neurons in spared DRG in the operated side was significantly less than that of normal group(P<0.05), but significantly more than that of L 6 DRG in the unoperated side(P<0.05); the number of NT-4 positive neurons of L 6 DRG on the 14 th day after partial dorsal root rhizotomy was also less than that of normal group, 7-day group, and the un-operated side(P<0.05).(2) There was no difference in respect to the number of NT-4 positive neuron in L 3, L 5, L 6 spinal lamina Ⅱand L 3 Clarke's nucleus in the operated side between the normal group, 7-day operation group and 14-day operation group(P>0.05). Conclusion Our results suggest that partial dorsal root rhizotomy can mainly lead to the change of NT-4 expression in the spared root ganglion, and NT-4 in the unoperated side DRG are inv

关 键 词：神经生物学 部分背根切断 免疫组化 猫 神经营养因子-4 

分 类 号：R651[医药卫生—外科学]