维生素D受体基因多态性与汉族人群慢性牙周炎易感性的关系  

Association of vitamin D receptor gene polymorphisms with the susceptibility to chronic periodontitis of Han nationality

作　　者：章锦才[1] 耿华欧[2] 马文波[3] 黄萍[3] 庞如愚[1] 张蕴惠[3] 

机构地区：[1]广东省口腔医院牙周科,广州510280 [2]武汉大学口腔医学院牙周科 [3]四川大学华西口腔医学院牙周科

出　　处：《中华口腔医学杂志》2005年第1期50-53,共4页Chinese Journal of Stomatology

摘　　要：目的　探讨维生素D受体 (VDR)基因多态性与汉族人群慢性牙周炎 (CP)易感性的关系。方法　收集汉族轻、中、重度CP患者共 16 6例及 80名无牙周炎对照者的颊黏膜拭子 ,以Chelex 10 0法提取DNA ,采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)方法测定VDRBsmI、VDRApaI和VDRTaqI的基因型 ,分析组间基因型分布和等位基因频率的差异。结果　轻、中、重度CP患者中VDRApaI等位基因A携带者明显多于对照组 ,重度CP与中度CP、重度CP与轻度CP患者间VDRApaI基因型的分布差异均有统计学意义 ,中度CP与轻度CP患者间VDRApal基因型分布差异无统计学意义 ,而VDRBsmI、TaqI位点的基因型分布在患者组和对照组间差异无统计学意义。Objective To investigate association of vitamin D receptor (VDR) gene polymorphisms with the susceptibility to chronic periodontitis (CP) of Han Nationality.Methods Buccal swabs from 166 patients with severe, moderate and mild CP respectively and 80 matched control individuals were collected. DNA was extracted from these buccal swabs using Chelex 100 method. VDR BsmI, ApaI, TaqI were tested with polymerase chain reaction restriction fragment length polymorphism (PCR RFLP). The distribution of the genotypes and allele frequencies in the patient and control groups were analyzed.Results The frequency of VDR ApaI allele A was significantly higher among patients with CP than controls. Frequencies of VDR ApaI allele A were significantly higher in severe CP patients than in moderate CP and mild CP respectively. There was no significant difference in the genotype distribution or the allele frequencies of VDR BsmI and TaqI between the controls and CP patients.Conclusions These data indicate that VDR ApaI allele A may be related to the susceptibility to CP in Han Nationality.

关 键 词：CP 患者 易感性 重度 基因型分布 慢性牙周炎 维生素D受体基因多态性 汉族人群 VDR DNA 

分 类 号：R781.4[医药卫生—口腔医学]