金雀异黄素诱导人乳腺癌细胞凋亡作用机制研究  

Effect of apoptosis in human breast cancer cells and its probable mechanisms by genistein

作　　者：刘颖[1] 张玉梅[2] 宋丹凤[3] 崔洪斌[3] 

机构地区：[1]哈尔滨商业大学生物工程系,哈尔滨150076 [2]北京大学公共卫生学院 [3]哈尔滨医科大学公共卫生学院

出　　处：《卫生研究》2005年第1期67-69,共3页Journal of Hygiene Research

基　　金：国家自然科学基金项目(No.30100151);黑龙江省自然科学基金项目(No.D0124)

摘　　要：目的探讨金雀异黄素对体外培养的人乳腺癌MCF7细胞凋亡的影响及作用机制。方法采用MMT法、凋亡细胞的荧光显微镜、电镜观察和流式细胞仪的检测,观察了不同剂量的金雀异黄素诱导细胞凋亡。同时采用免疫组化法检测凋亡相关基因Bax和癌基因erbB2蛋白的表达。结果通过荧光显微镜和电镜技术观察到凋亡的MCF7细胞。流式细胞仪也检测到了金雀异黄素诱导MCF7细胞产生凋亡,并随着金雀异黄素浓度的增加,细胞凋亡率显著增加。蛋白水平检测表明金雀异黄素促进MCF7细胞Bax表达升高,抑制erbB2表达。结论金雀异黄素可诱导MCF7细胞凋亡,并主要是通过调节Bax和erbB2蛋白表达实现的。Objective To investigate the effects of genistein on human breast cancer cell MCF-7 apoptosis and its probable mechanisms. Methods In this study, the methods of MTT, cell apoptosis detecting in fluorescent and electronic microscope and flow cytometry, and expression of Bax and erbB-2 protein were employed. Results The results showed that genistein could significantly inhibit the growth and induce the apoptosis of MCF-7 cells. Apoptotic cells of morphology from MCF-7 cells treated by different concentrations of genistein were observed by fluorescent and electronic microscope and the frequency of apoptosis in MCF-7 cells by flow cytometry showed increasingly with concentrations of genistein increased. The expression of Bax protein in MCF-7 cells was increased and the expression of erbB-2 protein was decreased with the doses of genistein. Conclusion Genistein can induce MCF-7 cells apoptosis and it may be one of the mechanisms for the inhibitory effect of genistein in human cancer cells.

关 键 词：金雀异黄素 乳腺癌细胞 细胞凋亡 基因表达 

分 类 号：R737.9[医药卫生—肿瘤] Q51[医药卫生—临床医学]