PLC对ADP诱导的兔血小板cAMP的影响  

Effect of PLC on rabbit platelet cAMP induced by ADP

作　　者：王常高[1,2] 杨芳[3] 陈明锴[4] 干信[5] 何东平[3] 陈涛[3] 

机构地区：[1]中国科学院武汉病毒研究所 [2]湖北工业大学生物工程学院,湖北武汉430068 [3]武汉工业学院食品科学与工程学院 [4]武汉大学人民医院 [5]湖北工业大学生物工程学院

出　　处：《中国药理学通报》2005年第1期45-48,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金 (No30171074 );中国科学院创新工程重大项目(Nokscx1 sw 11)资助课题

摘　　要：目的　测定磷酯酶C(phospholipaseC,简称PLC,下同)对ADP诱导的兔血小板cAMP的影响,从而进一步阐明PLC抗血小板聚集作用的机制。方法　取家兔的PRP,除去红细胞,分别用生理盐水、ASA和不同剂量的PLC处理,以ADP诱导激活,采用三氯醋酸法制备血小板cAMP,再用放射免疫分析法分别测定cAMP含量。结果　家兔血小板经生理盐水处理后的cAMP含量为 ( 20 16±0 91 ) pmol/109platelets,而经生理盐水、ASA668μmol·L-1、5、10、15、20和25UPLC·ml-1各组处理,并以ADP激活的血小板,测得的cAMP含量分别为 13 85±1 14, 16 27±0 36, 18 74±0 55,19 80±0 52, 20 49±0 43, 22 55±0 61和 24 24±0 85(pmol/109 platelets)。以生理盐水作为对照,ASA668μmol·L-1、5、10、15、20和 25UPLC·ml-1剂量组对激活状态下血小板cAMP的含量有增加的作用,增加率 (% )分别为17 47, 35 31, 42 96, 47 94, 62 82, 75 02。结论　PLC使得ADP激活后的兔血小板cAMP水平提高(P<0 01),且呈剂量依赖性,表明PLC具有提高血小板中cAMP水平的作用。这可能是PLC抗血小板聚集作用的重要原因之一。Aim To study the effect of PLC on rabbit platelet cAMP , and then to explore the mechanism of PLC anti-aggregation to platelet. Me thod Rabbit platelets removed of red cells were treated with PSS, ASA an d different dose of PLC respectively and induced by ADP respectively. cAMP was extracted by trichloroacetic acid.The concentration of cAMP of differently trea ted platelets was determined by RIA (radioimmunoassay). Result When rabbit platelets were treated with PSS, the concentration of cAMP at static state was 20.16±0.91 pmol/109 platelets; when the platelets were treated w ith PSS、ASA 668 μ mol·L -1,5,10,15,20 and 25 U PLC·ml -1, the conc entration of cAMP of determined at activated state induced by ADP was 13.85±1 .14, 16.27±0.36,18.74±0.55, 19.80±0.52, 20.49±0.43, 22.55±0.61 and 24.24±0.85(pmol/109 platelets) respectively. Compared with PSS, enha nced rates ( % ) of ASA 668 μmol·L -1, 5, 10, 15, 20, 25 U PLC·ml -1 to the concentration of cAMP were 17.47, 35.31, 42.96, 47.94, 62.82, 75 .02 respectively. Conclusion PLC has significant effects on the concentration of cAMP of rabbit platelets(P<0.01), and increases cAMP dos e-dependantly(P<0.01). All these indicate PLC can enhance the concentrati on of cAMP. This may be one of the important reason why PLC can inhibit platele t aggregation.

关 键 词：PLC 血小板 CAMP 抗血小板聚集 

分 类 号：R-332[医药卫生] R331.143