L-精氨酸发酵动力学特性的分析研究  

Kinetic Study and Modelling on L-arginine Fermentation

作　　者：龚建华[1] 丁久元[1] 黄和容[1] 陈琦[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《生物工程学报》1993年第1期8-15,共8页Chinese Journal of Biotechnology

基　　金：国家自然科学基金

摘　　要：在2.6L自控小罐上用突变株971.1进行了L-精氨酸分批发酵,对涉及细胞群体代谢速率和代谢效率的发酵动力学行为进行了分析研究,内容有:(1)分析了该发酵过程的动力学类型,并提出了定量分类的'生长比速率双相比较法';(2)在实验基础上组建了L-精氨酸发酵的数学模型;(3)通过对不同溶氧系数k__(Lα)条件下的菌体生长耗底物分数α_x、产物生成耗底物分数α_y的分析比较,讨论了氧的供给对底物转化成L-精氨酸效率的影响。The batch culture of the L-arginine fermentation by Coorynebaoterium crenatum L-arginine-producing mutant 971.1 was kineticly studied in the 2.6 L fermenter for the purpose as follows: (1) Kinetic classification of L-arginine fermentation by means of the comparison of the two-phases of specific growth rate; (2) Modelling of the L-arginine fermentation; (3) The discussion about the effects of the oxygen supply on the conversion rate of glucose to L-arginine, by comparison between the fractions of glucose-consumption for L-arginine formation, α_(?), and the ones of glucose consumption for cell growth, α_x, under the different oxygen transfer coefficients, k_La. As a result, the kinetic fermentative behaviour of the strain 971.1, which relates to the velocity and the effectiveness of the metabolism of the cell, in L-arginine-formation cultivation were mathematically analysied.

关 键 词：L精氨酸 发酵动力学 数学模型 

分 类 号：Q81[生物学—生物工程]