Peplomycin induces G1-phase specific apoptosis in liver carcinoma cell line Bel-7402 involving G_2-phase arrest  

作　　者：Hong-yingCHE Cong-yiZHENG Guo-linZOU Da-xingXIE Jian-pingGONG 

机构地区：[1]CollegeofLifeSciences,WuhanUniversity,Wuhan430072 [2]CancerResearchInstitute,TongjiHospital,TongiiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430072,China

出　　处：《Acta Pharmacologica Sinica》2004年第12期1698-1704,共7页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (No 39770200; 39730270).

摘　　要：AIM: To investigate the mechanism of peplomycin (PEP)-induced apoptosis in liver carcinoma cell line (Bel-7402). METHODS: Growth inhibition by PEP was analyzed using 3- 4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptotic cells were detected using Hoechest 33258 staining, and confirmed by flow cytometric analysis and DNA fragmentation analysis. The expression of cyclin A and B1 were determined by flow cytometry and Western blot. Annexin V assay was measured by flow cytometric analysis. RESULTS: PEP induced apoptosis and then inhibited cell proliferation in liver carcinoma cell line Bel-7402. Cells treated with PEP 50 μmol/Lfor 15 h were arrested in G2-phase with dramatical expression of cyclin A and a little change in cyclin B1. Almost all the apoptosis occurred in cells undergoing the G1-phase after treatment for 24 h. CONCLUSION: Peplomycin induced G1-phase specific apoptosis in Bel-7402 involving G2-phase arrest.AIM: To investigate the mechanism of peplomycin (PEP)-induced apoptosis in liver carcinoma cell line (Bel-7402). METHODS: Growth inhibition by PEP was analyzed using 3- 4,5-Dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptotic cells were detected using Hoechest 33258 staining, and confirmed by flow cytometric analysis and DNA fragmentation analysis. The expression of cyclin A and B1 were determined by flow cytometry and Western blot. Annexin V assay was measured by flow cytometric analysis. RESULTS: PEP induced apoptosis and then inhibited cell proliferation in liver carcinoma cell line Bel-7402. Cells treated with PEP 50 μmol/Lfor 15 h were arrested in G2-phase with dramatical expression of cyclin A and a little change in cyclin B1. Almost all the apoptosis occurred in cells undergoing the G1-phase after treatment for 24 h. CONCLUSION: Peplomycin induced G1-phase specific apoptosis in Bel-7402 involving G2-phase arrest.

关 键 词：PEPLOMYCIN Bel-7402 cells APOPTOSIS CYCLINS cell cycle flow cytometry 

分 类 号：R735.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]