脂肪肝胰岛素抵抗大鼠模型的建立  

Establishing a rat insulin-resistant fatty liver model

作　　者：陈世清[1] 刘杞[1] 孙航[1] 张桂灵[1] 石小枫[1] 

机构地区：[1]重庆医科大学病毒性肝炎研究所,重庆医科大学附属第二医院,教育部感染性分子生物学重点实验室,400010

出　　处：《中华肝脏病杂志》2005年第2期105-108,共4页Chinese Journal of Hepatology

摘　　要：目的 建立脂肪肝胰岛素抵抗大鼠模型。 方法 雄性Wistar大鼠14只,随机分为模型组和正常组,模型组喂养高脂饮食,正常组喂养普通饮食,两组脂肪分别占摄入能量的45％和19％,共饲养8周。胰岛素敏感性用正常血糖高胰岛素钳夹技术稳态时的葡萄糖输注率(GIR)来评价,生物化学法测定氨基转移酶、甘油三酯(TG)、游离脂肪酸(FFA)、丙二醛(MDA)、超氧化物歧化酶(SOD)和放射免疫法测定胰岛素,并用光镜和电镜进行组织学和超微结构检查。 结果 模型组大鼠肝细胞均呈现弥漫性脂肪变性、肝小叶内炎症及线粒体异常,而正常组肝脏无异常。且模型组的氨基转移酶、TG、FFA和肝指数均显著高于正常组,血清胰岛素升高,GIR明显低于正常组,肝脏MDA升高、SOD降低。 结论 通过8周高脂饮食成功地建立脂肪肝胰岛素抵抗大鼠模型,为脂肪肝发病机制和治疗研究提供了一个理想的实验工具。Objective To create a rat insulin resistant fatty liver model. Methods 14 male Wistar rats were randomly divided into a model and a control group. The model rats were fed a high-fat diet (45% of energy from fat) for 8 weeks, and the control group a standard diet (19% of energy from fat). Insulin sensitivity was measured with glucose infusion rate (GIR) by the euglycermic hyperinsulinemia clamp technique. The aminotransferase, triglyceride (TG), free fatty acid (FFA), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured by biochemical methods, and insulin was measured by radioimmunoassay. The histological and ultrastructural changes of all rat livers were scored using light and electron microscopy. Results Rats fed the high-fat diet developed panlobular macrovesicular steatosis, lobular inflammatory cell infiltration and abnormal mitochondria, whereas those fed the standard diet had normal livers. All model group rats had elevated levels of aminotransferase, TG, FFA, insulin and liver index, and low GIR. In addition, the high-fat diet increased MDA and decreased SOD. Conclusion A fatty liver and insulin resistance model was successfully developed in rats fed a high-fat diet for 8 weeks, which provided a useful experimental tool for elucidating pathogenesis and treatment of fatty liver.

关 键 词：正常 脂肪肝 大鼠模型 胰岛素抵抗 高脂饮食 FFA 升高 氨基转移酶 钳夹技术 雄性 

分 类 号：R-332[医药卫生]