高效液相色谱测定鸟氨酸脱羧酶活性方法的改进  

Improvement for the high-performance liquid chromatography assay for the activity of ornithine decarboxylase

作　　者：魏凯[1] 李恩中[1] 李超坤[1] 许晓军[1] 宁黔冀[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《河南师范大学学报（自然科学版）》2004年第4期138-140,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省自然科学基金(20011800009);河南省高校青年骨干教师资助计划资助项目

摘　　要：对柴彪新等报道的高效液相色谱测定鸟氨酸脱羧酶(ODC)活性的方法作了改进.结果表明,改进后的方法更灵敏,更简便,线性范围明显扩大,相关系数为0.9998,显著高于柴彪新等报道的0.9939.同时,新方法还可使苯甲酸和苯甲酰氯降到很低.The HPLC method reported by Chai Biao-xin et al for determination of the activity of ornithine decarboxylase was improved. Results indicated that the promoted method was more sensitive and simply for ODC assay. The linear rang was apparently extended and the correlation coefficient was up to 0.9998 which was higher than Chai Biao-xin's 0.9939. The ODC activity in 10 partially hepatectomized rats liver was mensurated by using this promoted method, and it was significantly higher than that of control group.

关 键 词：高效液相色谱(HPLC) 方法改进 鸟氨酸脱羧酶 腐胺 

分 类 号：Q557.3[生物学—生物化学] R730.43[医药卫生—肿瘤]