深低温冷冻对rhBMP-2诱导犬气管移植体软骨再生的影响  

Effect of cryopreservation on rhBMP-2 induced regeneration of dog's tracheal cartilage

作　　者：闫小龙[1] 李小飞[1] 刘勇[1] 卢强[1] 王律[1] 

机构地区：[1]第四军医大学唐都医院胸外科,陕西西安710038

出　　处：《第四军医大学学报》2005年第2期160-163,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目(39970707)

摘　　要：目的:研究应用重组人骨形态发生蛋白 2(rhBMP 2)/胶原缓释系统诱导犬冷冻气管移植软骨再生的作用. 方法:将32只杂种犬随机等分为4组,行5个颈部气管环原 位异体移植,带血管蒂大网膜包绕.A组为未冷冻组,气管环 间植入rhBMP 2/胶原组标记为A1组,空白对照标记为A2 组;B组术前将移植体气管经深低温液氮保存2mo,处理同A 组,分别标记为B1、B2两组.所有实验犬于术后8wk处死,行 大体及病理学检查,评测各组新生软骨面积.结果:B组犬移 植气管结构保持较好、狭窄程度低.4组均有软骨再生,B1组 新生软骨面积1835.9(527.2)高于A1组1408.5(492.6)及 B2组336.8(121.3),各组新生软骨面积差异有显著性(χ2= 24.010,df=3,P<0.01).结论:应用rhBMP 2可刺激冷冻 气管移植体软骨再生,其刺激深低温冷冻犬气管移植体成骨 效果优于未冷冻气管移植体.AIM: To confirm the validity and potential application of implanting the recombinant human bone morphogenetic protein-2 (rhBMP-2) with collagen into the cryopreserved tracheal allografts to induce the regeneration of dog's tracheal cartilage. METHODS: Thirty-two mongrel dogs were randomly and equally divided into 4 groups. Five-ring cervical tracheal segment was harvested as the alloraft and all the grafts were wrapped with pedicled omentum. Group A was subdivided into group A1, in which RhBMP-2 with collagen as a carrier was injected into the soft tissues between cartilaginous rings of the grafts, and group A2 --the blank group. Group B, in which the grafts were cryopreserved for two months before the operation, was also subdivided into group B1 and group B2. The animals were sacrificed 8 weeks postoperation. The postmortem specimens were examined grossly and histologically. Area of new cartilage was studied in each allografts. RESULTS: Structural integrity of the allografts was better in group B than in group A. In all the groups, new cartilage was induced in the rhBMP-2 injected area and more new cartilage was observed in group B1 than in group A1. CONCLUSION: rhBMP-2 has the ability to induce new cartilage formation in the cryopreserved racheal allografts. Cryopreserved tracheal allografts have better new cartilage inducing effect than noncryopreserved tracheal allografts.

关 键 词：气管 移植 低温保藏 移植物排斥 骨形态发生蛋白质类 

分 类 号：R734.2[医药卫生—肿瘤]