生物制药研究中的多维色谱  

Multidimensional Separations Used in Pharmaceutical and Biological Fields

作　　者：田晶[1] 路鑫[1] 杨军[1] 孔宏伟[1] 王媛[1] 赵欣捷[1] 唐平 苑广志[1] 许国旺[1] 

机构地区：[1]中国科学院大连化学物理研究所国家色谱研究分析中心

出　　处：《色谱》2005年第1期32-36,共5页Chinese Journal of Chromatography

基　　金：国家重点基础研究发展规划("973"规划)(2003CB716003);国家"863"计划(2003AA223061)资助项目.

摘　　要：对全二维气相色谱(GC×GC)、全二维液相色谱(HPLC×HPLC)、多维毛细管电泳等多维分离技术在生物制药研究中的应用进行了综述,其中对作者所在研究组在全二维气相色谱应用于中药及固相萃取 液相色谱联用分析系统等方面的工作做了重点介绍。由所综述的生物制药研究得出结论:多维分离方法以其高分辨、快速、自动化等特点已经在生物制药领域显示出它的巨大优势,并将发挥更大的作用。A review of multidimensional separations such as comprehensive two-dimensional gas chromatography (GC×GC), comprehensive two-dimensional high performance liquid chromatography (HPLC×HPLC) and their applications in pharmaceutical and biological fields is presented with 71 references. A single CO_2 cryo-jet loop modulator was developed for GC×GC and it can be used to modulate compounds higher than C_6 effectively. Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC/TOF-MS) analyses of traditional Chinese medicine volatile oils such as Pogostemon cablin Benth (Cablin Patchouli), Forsythia suspensa (Thunb.) Vahl and Zedoary were reported also. As an emerging technology, multidimensional separations hold the promise and play an important role in the future pharmaceutical and biological fields.

关 键 词：多维分离 色谱 联用技术 生物 制药 综述 

分 类 号：O658[理学—分析化学]