猪细小病毒VP2蛋白主要抗原表位基因真核表达载体的构建及表达  

Expression of the Domain Gene of Porcine Parvovirus VP2 with the Eukaryotic Vector pIREShyg

作　　者：任雪枫[1] 胡志华[1] 谈国蕾[1] 周斌[1] 徐学清[1] 郑其升[1] 张晓勇[1] 陈德胜[1] 陈溥言[1] 

机构地区：[1]南京农业大学动物医学院农业部动物疫病诊断与免疫重点开放实验室,江苏南京210095

出　　处：《中国病毒学》2004年第6期636-638,共3页Virologica Sinica

基　　金：国家"863"高技术发展计划资助项目(2001AA249012)。

摘　　要：Using a pair of specific primers designed according to the relevant nucleotide sequences from GenBank, the main antigen domain for VP2 gene of Porcine parvovirus was ampilified with PCR method using the genomic DNA as template. The PCR product was cloned into the expression vector pIREShyg to get a recombinant eukaryotic expression plasmid pIREShyg-VP2, which was then transfected into the CHO-K1 cells. The expressed product was detected by IFA after the positive cell clone was selected with hygromycin. The result revealed that the main antigen domain for VP2 gene of porcine parvovirus was stably expressed in CHO-K1 cells.Using a pair of specific primers designed according to the relevant nucleotide sequences from GenBank, the main antigen domain for VP2 gene of Porcine parvovirus was ampilified with PCR method using the genomic DNA as template. The PCR product was cloned into the expression vector pIREShyg to get a recombinant eukaryotic expression plasmid pIREShyg-VP2, which was then transfected into the CHO-K1 cells. The expressed product was detected by IFA after the positive cell clone was selected with hygromycin. The result revealed that the main antigen domain for VP2 gene of porcine parvovirus was stably expressed in CHO-K1 cells.

关 键 词：猪细小病毒 VP2蛋白 潮霉素 CHO-K1细胞 

分 类 号：S852.65[农业科学—基础兽医学]