人血浆第Ⅴ因子蛋白含量的免疫测定  

A NOVEL ENZYME IMMUNOASSAY FOR DETERMINATION OF HUMAN PLASMA FACTOR V

作　　者：谢飞[1] 兰风华[1] 朱晓辉[1] 唐玉钗[1] 朱忠勇[1] 

机构地区：[1]福州总医院全军医学检验中心

出　　处：《福州总医院学报》2000年第1期23-25,共3页Journal of Fuzhou General Hospital

摘　　要：目的建立一种双抗体夹心 ELISA,以测定人血浆第 V 因子(FV)抗原含量。方法采用兔抗人 FV 抗体为包被抗体,FV 单抗为检测抗体,首次对中国正常人(n=26)和一个遗传性 FV 缺乏家系的有关成员血浆中的 FV 抗原进行了检测。结果该法线性范围广、特异性强、灵敏度高、重复性较好,和 FV 活性测定法有很好的相关性;正常人血浆 FV 抗原星偏态分布:该家系纯合子血浆 FV 抗原量只有正常人的2%,证实该家系为Ⅰ型遗传性 FV 缺乏症。结论建立了一种新的 FV 蛋白定量测定法,该法可以对 FV 缺乏症进行辅助诊断和分型。Ob jective To establish a novel quantitative method for determination of human plasmafactor V.method A double-antibody sandwich ELISA using the rabbit anti-human FV antibody for platecoating and monoclonal antibody against FV as second antibody was applied to quantify FV antigen in plasmasfrom normal Chinese adults(n=26)and members of a hereditary FV deficiency family.Results This methodwas specific and sensitive with a wide linear range.It was in excellent correlation with the coagulantactivity assay.The distribution of FV concentration in plasmas from 26 normal subjects was skewed.Ahomozygote for hereditary FV deficiency had 2% of normal plasma FV concentration,indicating the presenceof a type Ⅰ factor Ⅴ deficiency.Conclusion The FV quantitative assay was useful for the diagnosis andclassification of FV deficiency.

关 键 词：人血浆 抗原 FV 正常人 家系 免疫测定 抗体 遗传性 首次 辅助诊断 

分 类 号：R749.13[医药卫生—神经病学与精神病学] R969.1[医药卫生—临床医学]