自絮凝酵母SPSC01在组合反应器系统中酒精连续发酵的研究  

Continuous Ethanol Fermentation Using Self-flocculating Yeast Strain and Bioreactor System Composed of Multi-stage Tanks in Series

作　　者：徐铁军[1] 赵心清[1] 周友超 白凤武[1] 

机构地区：[1]大连理工大学生物科学与工程系,大连116023 [2]安徽丰原生物化学股份有限公司,蚌埠233010

出　　处：《生物工程学报》2005年第1期113-117,共5页Chinese Journal of Biotechnology

基　　金：国家高技术研究与发展计划 (863 )项目基金资助 (No .2 0 0 2AA64 70 60 )。~~

摘　　要：建立了一套由四级磁力搅拌发酵罐串联组成、总有效容积 4 0 0 0mL的小型组合生物反应器系统 ,其中一级罐作为种子培养罐。以脱胚脱皮玉米粉双酶法制备的糖化液为种子培养基和发酵底物 ,进行了自絮凝颗粒酵母酒精连续发酵的研究。种子罐培养基还原糖浓度为 10 0g L ,添加 (NH4 ) 2 HPO4 和KH2 PO4 各 2 0g L ,以 0 0 17h- 1 的恒定稀释速率流加 ,并溢流至后续酒精发酵系统。发酵底物初始还原糖浓度 2 2 0g L ,添加 (NH4 ) 2 HPO4 1 5g L和KH2 PO4 2 5g L ,流加至第一级发酵罐 ,稀释速率分别为 0 0 17、0 0 2 5、0 0 33、0 0 4 0和 0 0 5 0h- 1 。实验数据表明 ,自絮凝颗粒酵母在各发酵罐中呈部分固定化状态 ,在稀释速率0 0 4 0h- 1 条件下 ,发酵系统呈一定的振荡行为 ,其他四个稀释速率实验组均能够达拟稳态。当稀释速率不超过 0 0 33h- 1 ,流出末级发酵罐的发酵液中酒精浓度可以达到 12 % (V V)以上 ,残还原糖和残总糖分别在 0 11%和 0 35 % (W V)以下。在稀释速率为 0 0 33h- 1 时 ,计算发酵系统酒精的设备生产强度指标为 3 32 (g·L- 1 ·h- 1 ) ,与游离酵母细胞传统酒精发酵工艺相比 ,增加约 1倍。A continuous ethanol fermentation system composed of four_stage tank fermentors in series and with a total working volume of 4000 mL was established. The first fermentor was designated as the seed fermentor and the others for ethanol fermentation. A self_flocculating yeast strain developed by protoplast fusion of Saccharomyces cerevisiae and Schizosaccharomyces pombe was applied. Two_stage corn powder enzymatic hydrolyzate containing reducing sugar 100 g/L, together with 2 0g/L (NH 4 ) 2 HPO 4 and KH 2 PO 4 , was used as yeast seed culture medium and fed into the seed fermentor at the dilution rate of 0 017h -1 . Meanwhile, the hydrolyzate containing reducing sugar 220 g/L, added with 1 5g/L (NH 4 ) 2 HPO 4 and 2 5g/L KH 2 PO 4 , was used as ethanol fermentation substrate and fed into the second fermentor at the dilution rates of 0 017, 0 025, 0 033, 0 040 and 0 050 h -1 (based on the total working volume of the three fermentors), respectively. The chemostat states on which all of the monitoring parameters, including residual sugar, ethanol and yeast cell biomass concentrations, were maintained relatively constant were observed for seed cultivation and ethanol fermentations when the fermentation system was operated at the dilution rates of 0 017, 0 025, 0 033 and 0 050 h -1 . Yeast cells were observed being partly immobilized because significant yeast cell biomass concentration differences between the broth out of and inside the fermentors were detected. Moreover, the oscillations of residual sugar, ethanol and yeast cell biomass concentrations were observed when the fermentation system was operated at the dilution rate of 0 040 h -1 . The broth containing more than 12% (V/V) ethanol and less than 0 11% (W/V) residual reducing sugar and 0 35% (W/V) residual total sugar was produced when the dilution rate was controlled at no more than 0 033 h -1 . The ethanol productivity was calculated to be 3 32(g·L

关 键 词：自絮凝颗粒酵母 酒精连续发酵 振荡行为 多罐串联生物反应器系统 

分 类 号：TS262.2[轻工技术与工程—发酵工程]