D3S1358等10个短串联重复序列位点在亲子鉴定中的应用  

Study on the application of ten STR loci in parentage testing

作　　者：许业莉[1] 蔡颖[1] 相大鹏[1] 庄逸林[1] 梁希扬[1] 

机构地区：[1]汕头出入境检验检疫局,广东汕头515031

出　　处：《中国微生态学杂志》2005年第1期36-38,共3页Chinese Journal of Microecology

摘　　要：目的分析广东汕头地区汉族人群的D3S1358、vWA、D16S539、D2S1338、D8S1179、D21S11、D18S51、D19S433、TH01和FCA位点多态性,探索在该人群中联合应用这10个位点进行亲子鉴定的应用价值。方法利用4色荧光标记多重PCR和全自动毛细管电泳技术对短串联重复序列(STR)位点进行基因分型。结果统计分析241例无关个体10个位点的基因频率,所有位点均符合HWE。经计算,D2S1338、FGA、D18S51和D8S1179属于高度多态性位点,D19S433、D21S11、vWA和D16S539属于中高度多态性位点,而D3S1358、TH01多态性方面稍差。10个位点的个人识别能力(DP)、多态性信息总量(PIC)、杂合度(H)和非父排除概率(PE)各指标的累积值均>09999,平均偶合率为98×1013。在114例亲子鉴定中联合应用10个STR位点,7例排除亲子关系的排除指标不少于4个;可以肯定有亲子关系70例,亲子关系概率(RCP)均≥9990%;其余37例RCP<9990%,需要增加鉴定位点,才能得出结论。结论联合应用这10个STR位点,在该地区可成功地开展亲子鉴定,但仍有部分单亲亲子鉴定需要增加STR位点,以期降低误判的风险。Objective To analyze the genetic polymorphism of 10 STR loci which include D3S1358,vWA,D16S1338,D8S1179,D21S11,D18S51,D19S433,TH01 and FGA among Han population in Shantou and to explore the application of these STR loci in parentage testing. Methods The 10 STR loci were genotyped by four colors fluorescence label multiplex PCR and full automatic capillary electrophoresis.Results Allele frequencies of all the 10 STR loci of 241 unrelated individuals were calculated, and statistic analysis showed that their distribution all met Hardy-Weinberg equilibrium.The results suggested that four STR loci were high polymorphic, four STR loci were middle-high polymorphic. The combined DP,PIC,H and PE of the 10 STR loci were all greater than 0.9999 and the population match probability were 9.8×10^-13 .Among 114 cases of parentage testing, all of 7 cases with exclusive paternity were incoordinate with more than 4 loci.Among the 107 cases with determined paternal relation, the RCP in 70 cases were greater than 99.90%.Conclusion The 10 STR loci were suitable for parentage testing in Shantou,but in some duo paternity test cases other new STR loci must be test for smaller risk of making a wrong conclusion.

关 键 词：联合应用 多态性位点 位点 TH01 汕头地区 人群 基因分型 杂合度 累积 高度 

分 类 号：R394[医药卫生—医学遗传学] S512[医药卫生—基础医学]