金雀异黄酮对去卵巢大鼠基底前脑一氧化氮合酶神经元的影响  

Effects of genistein on nitric oxide synthase positive neurons of basal forebrain in ovariectomized rats

作　　者：朱建华[1] 郭开华[1] 徐杰[1] 

机构地区：[1]中山大学中山医学院人体解剖学教研室,广东省广州市510080

出　　处：《中国临床康复》2005年第5期36-37,共2页Chinese Journal of Clinical Rehabilitation

基　　金：广东省自然科学基金重点项目(4105332)~~

摘　　要：目的:研究去卵巢及金雀异黄酮(genistein,GS)治疗后基底前脑一氧化氮合酶(nitricoxidesynthase,NOS)阳性神经元数目变化,探讨GS改善去卵巢大鼠学习记忆能力的可能机制,为使用GS防治绝经后老年性痴呆提供理论依据。方法:实验于2003-03/2004-01在中山大学人体解剖学教研室脑研究室进行。将36只3月龄雌性SD大鼠分为假手术组、去卵巢对照组、苯甲酸雌二醇组及金雀异黄酮组,每组9只,分别进行干预。术后8周取脑切片,进行NADPH-d组化方法染色,计数分析基底前脑各区NOS阳性神经元数目。结果:各组大鼠基底前脑内侧隔核NOS阳性神经元数目差异无显著性意义(P>0.05);去卵巢对照组斜角带核垂直支及水平支的NOS阳性神经元数目分别为(33.13±9.80)个和(48.55±6.60)个,均高于假手术组、苯甲酸雌二醇组及金雀异黄酮组(P<0.01),金雀异黄酮组斜角带核垂直支及水平支的NOS阳性神经元数目与假手术组、苯甲酸雌二醇组相比差异均无显著性意义(P>0.05)。结论:去卵巢之后大鼠基底前脑一氧化氮增加,产生神经元的毒性作用,使中枢神经系统退行性变导致大鼠学习记能力下降;GS能减少基底前脑一氧化氮水平,有望替代雌激素用于防治疗女性绝经期后中枢神经系统退行性疾病。AIM:To investigate the numeral changes of nitric oxide synthase(NOS) positive neurons in basal forebrain after ovariectomy and hormone replacement therapy with genistein(GS),and probe into the possible mechanism of GS in ameliorating the learning and memory ability of ovariectomized rats,so as to provide basic theories for preventing and treating postmenopausal Alzheimer disease(AD) with GS. METHODS:The experiment was completed in the brain research room of Department of Human Anatomy,Sun Yat sen University from April to August 2004.Thirty six female Sprague Dawley rats of 3 month old were randomly divided into 4 groups with 9 rats in each:sham operated group,ovariectomized control group,estradiol benzoate group and GS group.The rats were sacrificed 8 weeks after operation,and the brain sections were stained with NADPH diaphorase histochemistry, and the positive neurons of basal forebrain were counted and statistically analyzed. RESULTS:There was no remarkable difference of NOS positive neurons in the inside septal nuclei of basal forebrain among each group(P >0.05). The numbers of NOS positive neurons in vertical diagonal band(VDB) and horizontal diagonal band(HDB) of the ovariectomized control group(33.13±9.80, 48.55±6.60) were markedly higher than those in the sham operated group, estradiol benzoate group and GS group(P< 0.01). The number of NOS positive neurons in VDB and HDB of the GS group has insignificant differences from those in the sham operated group and estradiol benzoate group(P >0.05). CONCLUSION:The nitric oxide(NO) in the basal forebrain of rats increases after ovariectomy.Neurotoxicity can lead to neurodegeneration of central nervous system(CNS),which will result in the impairment of learning and memory abilities in ovariectomized rats.GS can decrease the NO level in the basal forebrain of ovariectomized rats,and may be used as a replacement of estrogen to prevent and cure postmenopausal neurodegenerative disease of CNS.

关 键 词：异黄酮类 一氧化氮合酶 前脑 学习 记忆 

分 类 号：R74[医药卫生—神经病学与精神病学]