质粒pcDNA3.1-asHpa的构建及意义  

Construction of pcDNA3.1-asHpa and its significance

作　　者：阮绪芝 黄立英[2] 李瑞明 陈霞平 

机构地区：[1]湖北省郧阳医学院附属太和医院生命科学研究所,十堰442000 [2]中山大学达安基因公司

出　　处：《陕西医学杂志》2005年第2期138-140,共3页Shaanxi Medical Journal

基　　金：湖北省教委科研发展基金资助项目 [编号 (2 0 0 1 ) 0 2 2号 ]

摘　　要：目的 :构建乙酰肝素酶信号密码的反义基因真核表达载体 ,探索恶性肿瘤基因治疗的新途径。方法 :以质粒 pc DNA3.1为载体 ,将乙酰肝素酶信号密码的 DNA片段反向插入其多克隆位点得到反义乙酰肝素酶信号密码真核表达载体 ( pc DNA3.1 - as Hpa)。结果 :经过 PCR扩增鉴定、电泳证实获得的重组基因的大小和插入方向正确。结论 :成功的构建了乙酰肝素酶信号密码的反义基因真核表达载体 。Objective:To construct of human antisense heparanase signal code gene (pcDNA3.1-asHpa) and explore a new method in tumor therapy. Methods: Human heparanase signal code DNA were inserted into the expression plasmid pcDNA3.1 site in an antisense orientation to produce pcDNA3.1-asHpa. Results: The PCR and electrophoresis were carried out,and the antisense expression vector was verified. Conclusion: Human antisense heparanase signal code gene pcDNA3.1 vector has been constructed and could be used for related study of antisense gene expression.

关 键 词：PCDNA3 乙酰肝素酶 反义基因 质粒 治疗 瘤基因 真核表达载体 DNA片段 克隆 电泳 

分 类 号：R730[医药卫生—肿瘤] R735[医药卫生—临床医学]