镉对白菜活性氧代谢及H_2O_2清除系统的影响  

Effects of Different Cadmium Levels on Active Oxygen Metabolism and H_2O_2-Scavenging System in Brassica campestris L.ssp.Chinensis

作　　者：孙光闻[1,2] 朱祝军[1] 方学智[1,3] 

机构地区：[1]浙江大学园艺系 [2]华南农业大学园艺学院,广州510642 [3]中国林业科学研究院亚热带林业研究所,富阳311400

出　　处：《中国农业科学》2004年第12期2012-2015,共4页Scientia Agricultura Sinica

摘　　要：研究不同浓度镉对白菜(Brassica campestris L.ssp.chinensis)叶片活性氧产生及H2O2清除酶活性影响。结果表明,培养液中加镉增加了白菜叶片中超氧自由基(O2-)的产生速率和H2O2含量,过氧化产物丙二醛(MDA)含量随培养液中镉浓度和镉处理时间增加而增加。在加镉条件下,抗坏血酸过氧化物酶(APX)活性、脱氢抗坏血酸还原酶(DR)和谷胱甘肽还原酶(GR)活性升高,抗坏血酸(AsA)和谷胱甘肽(GSH)含量增加。The effects of different Cd levels on generation of active oxygen speceies (AOS) and H2O2-scavenging system in the leaves of Brassica campestris L.ssp.chinensis were studied. The results showed that the addition of Cd in the nutrient solution increased O2-?generation rate and H2O2 content. Malondialdehyde (MDA) content increased with the increase of Cd concentrations in the culture medium and treatment time. The activities of ascorbate peroxidase (APX), dehydroascorbate reductase (DR) and glutathione reductase (GR) were promoted by the addition of Cd, and the contents of ascorbate and glutathione were also increased when exposed to Cd.

关 键 词：白菜 活性氧代谢 产生速率 培养液 叶片 浓度 抗坏血酸过氧化物酶 含量 GR 影响 

分 类 号：S634.3[农业科学—蔬菜学]