遗传性P77PMC癫痫大鼠海马基因表达谱的研究  

The gene expression patterns of genetic P77PMC epilepsy prone rats' hippocampus

作　　者：吴志国[1] 肖波[1] 杨晓苏[1] 唐发清[2] 曾艺[1] 谢光洁[1] 

机构地区：[1]中南大学湘雅医院神经内科,长沙410008 [2]中南大学湘雅医院中心实验室,长沙410008

出　　处：《中华医学遗传学杂志》2004年第6期619-621,共3页Chinese Journal of Medical Genetics

基　　金：教育部高等学校骨干教师资助计划项目(2000-65)~~

摘　　要：目的利用cDNA表达阵列构建遗传性癫痫大鼠海马基因表达谱,寻找其中的差异表达基因,为从分子水平探讨癫痫的发病机理打下基础。方法采用32P-α-dATP逆转录标记探针与cDNA阵列杂交,构建P77PMC大鼠和Wistar大鼠海马基因表达谱,用图象分析仪分析两者基因表达谱差异。结果在P77PMC大鼠海马中共发现有15个差异表达基因,其中12个基因表达上调,3个基因表达下调。并用逆转录-聚合酶链反应进一步证实了结果的可靠性。结论P77PMC大鼠与正常Wistar大鼠海马中存在多个差异表达基因,这些差异表达的基因可能在癫痫的发生中具有重要作用。Objective To find out the differentially expressed genes in the hippocampus of the rats with genetic epilepsy so as to lay a foundation for exploring the pathogenesis of epilepsy by means of cDNA array technology. Methods Gene expression patterns in the hippocampus of the genetic epilepsy prone P77PMC rats and normal Wistar rats were established using the α 32 P labeled cDNA probes hybridized with the Atlas TM Rat cDNA Expression Array, and then were analyzed by an image analysis instrument to get the differentially expressed genes. Results Fifteen genes were found having differential expression patterns in hippocampus between the P77PMC rats and the Wistar rats, while there may be many other differentially expressed genes left undiscovered due to having no appropriate image analysis software. And among the fifteen genes, the expression levels of twelve genes in the P77PMC rats were higher than those in the Wistar rats, while the expression levels of the other three genes were lower. The results of reverse transcription polymerase chain reaction(RT PCR) have demonstrated the reliability of cDNA arrays method. Conclusion cDNA array is a powerful tool for identifying differential expression genes of epilepsy on large scales. There are several differentially expressed genes in hippocampus of the P77PMC rats and the Wistar rats. All these identified genes could play potentially important roles in the pathogenesis of epilepsy.

关 键 词：基因表达谱 大鼠海马 癫痫 差异表达基因 WISTAR大鼠 遗传性 cDNA表达阵列 ATP 逆转录 探针 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R743.31[医药卫生—临床医学]