双色荧光原位杂交检测苯系物接触者精子7、8号染色体数目畸变  

Detection of numerical aberrations of chromosomes 7 and 8 in sperms of workers exposed to benzene series by two-color fluorescence in situ hybridization

作　　者：赵婷[1] 刘新霞[1] 何云[1] 邓丽霞[1] 郑履康[1] 

机构地区：[1]中山大学公共卫生学院遗传毒理研究室,广州510089

出　　处：《中华医学遗传学杂志》2004年第4期360-364,共5页Chinese Journal of Medical Genetics

基　　金：广东省自然科学基金 (0 0 1 334)~~

摘　　要：目的　检测苯系物接触者精子 7、8号染色体数目畸变率。方法　用生物素标记 7号染色体探针 (D7Z1)和地高辛标记 8号染色体探针 (D8Z1)与精子核进行双色荧光原位杂交 ,测定精子染色体畸变率。结果　车间空气中苯的时间加权浓度为 42 .2 9mg/m3 ,高于国家卫生标准 ( 6mg/m3 )。苯系物接触者尿粘康酸浓度 ( 0 .861mg/g肌酐 ) ,高于对照组 ( 0 .44 4mg/g肌酐 )。杂交效率 99.77%～ 99.97% ,共计数15例苯系物接触者 15 5 72 1条精子核和 12名对照组者 12 3 771条精子核型。接触组二倍体精子率 ,平均7、8号染色体双体精子率 (分别为 0 .12 9%、0 .170 %和 0 .0 78% )均高于对照组 (分别为 0 .0 5 5 %、0 .0 5 3 %、0 0 3 3 % )。接触组 7号、8号染色体缺体精子率 (分别为 0 .165 %和 0 .0 88% ) ,也分别高于对照组 ( 0 .0 5 6%、0 0 2 9% )。总数目畸变精子率接触组为 0 .745 % ,高于对照组的 0 .2 89%。结论　较高浓度的苯系物暴露可引起接触者精子 7、8号染色体非整倍体率和二倍体精子率增高。Objective To investigate the frequency of numerical aberrations for chromosomes 7 and 8 in the sperms of workers exposed to benzene series. Methods Numerical aberrations in the sperms of workers were detected by two-color fluorescence in situ hybridization with biotin labeled chromosome 7 probe (D7Z1) and digoxingenin labeled chromosome 8 probe(D8Z1). Results The time-weight average air concentration(TWA) of benzene in the workshop was 42.29 mg/m3, which was significantly higher than that of the national maximum allowable concentration(6 mg/m3). The concentration of urinary trans,trans-muconic acid(ttMA) in the exposed group was also higher than that of the control group. In all, 155 721 sperm nuclei from 15 workers in the exposed group and 123 771 sperm nuclei from 12 individuals in the control group were examined. The results showed that the frequency of diploidy sperms and the frequencies of disomic sperm for chromosomes 7 and 8 in the exposed group (0.129%, 0.170%, 0.078%) were significantly higher than those of the control group (0.055%, 0.053%, 0.033%), respectively. The frequencies of nullisomic sperm for chromosomes 7 and 8 in the exposed group (0.165%, 0.088%) were also significantly increased, compared with those of the control group (0.056%, 0.029%). A statistically significant difference in the frequency of overall numerical chromosome aberrations was seen between the exposed group (0.745%) and the control group (0.289%). Conclusion The results suggested that higher concentration of benzene may induce higher frequencies of numerical aberrations in the sperms of workers exposed to benzene series.

关 键 词：精子 8号染色体 接触者 对照组 双色荧光原位杂交 苯系物 肌酐 数目 畸变率 地高辛标记 

分 类 号：R394[医药卫生—医学遗传学]