利用农业废弃物生产嗜热真菌(T.lanuginosus)耐热木聚糖酶的固体发酵研究  

Solid state fermentation for producing thermostable endoxylanase by T. lanuginosus using agricultural wastes

作　　者：李秀婷[1] 杨绍青[1] 江正强[1] 李里特[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《工业微生物》2004年第4期13-18,共6页Industrial Microbiology

基　　金：国家"十五"攻关课题 ( 2 0 0 1BA70 8B0 4 0 6)的部分内容

摘　　要：研究了嗜热真菌 (ThermomyceslanuginosusCBS2 88.5 4 M 18)生产木聚糖酶的碳氮源组成、料水比、培养基初始 pH值、发酵温度及接种量等的影响。结果表明 ,以麦麸和玉米芯粉 (8∶2 )作为复合碳源 ,酵母膏和胰蛋白胨 (1∶1)作为复合氮源时 ,菌株所产的木聚糖酶量相比未优化碳氮源的培养基提高幅度高达 2 0 0 %。其最佳产酶的料水比为 1∶3,培养基初始 pH 7.0为最适。菌株在 5 0℃条件下发酵 5d ,能够得到活力高达 15 0 2 3U g干基碳源的木聚糖酶制剂 。The cultivation of Thermomyces lanuginosus CBS288.54-M18 for the production of thermostable xylanase by solid state fermentation (SSF) was carried out. The effects of carbon and nitrogen sources, carbon source to mineral solution ratio, fermentation temperature and initial pH of culture on enzyme production were investigated. Results showed that, by using wheat bran and corncob at the ratio of 8:2 as the carbon source, yeast extract and tryptone at the ratio of 1:1 as the nitrogen source, amount of xylanase produced by the culture increased 200 % compared to those without optimization of carbon and nitrogen sources. The optimal carbon source to mineral solution ratio and the initial pH of cultures for enzyme production was 1:3 and pH 7.0, respectively. Under these conditions, the culture yielded cellulase-free and protease-free xylanase with activity of 15023 U/g of carbon source after fermented at 50 ℃ for 5 days.

关 键 词：培养基 料水比 碳氮源 玉米芯粉 酶制剂 木聚糖酶 菌株 初始PH值 固体发酵 碳源 

分 类 号：TQ929[轻工技术与工程—发酵工程]