p53基因测序在恶性肿瘤诊断中的应用价值观察  

Diagnostic Value of p53 Gene Sequencing in Clinical Common Neoplasm

作　　者：顾其华[1] 李玲芝[1] 舒畅[2] 杨志毅[1] 叶爱慧[1] 

机构地区：[1]衡阳医学院附属怀化医院,湖南怀化418000 [2]湖南医科大学湘雅医院

出　　处：《南华大学学报（医学版）》2000年第6期559-561,共3页Journal of Nanhua University(Medical Edition)

基　　金：湖南省卫生厅资助(97100);湖南省科委社会发展计划立项(98-SSN-3063).

摘　　要：目的　研究p53基因测序在临床肿瘤诊断中的应用价值。方法　采用聚合酶链反应 -直接测序方法对肺癌、胃癌、大肠癌、肝癌、乳腺癌、鼻咽癌、甲状腺癌、宫颈癌及支气管和胃粘膜非典型增生的活检标本 ,急、慢性白血病的外周血标本 ,肺癌胸水、肝癌腹水标本等临床肿瘤样本进行p53基因外显子 5、6、7、8序列分析。结果　正常对照组未发现突变 ,所测的几种常见肿瘤样本的活检标本的错义突变率一般为 30 %～ 50 % ,胸、腹水标本突变率与相应肿瘤的活检标本相近 ,突变大多位于外显子 7、8。存在突变的病例 2年病死率明显升高。Objective:To study the value of p53 gene sequencing in clinical diagnosis of common cancers.Methods:Polymerase chain reaction(PCR) and direct sequencing were used to analyse exons 5,6,7,8 of human p53 gene.Samples of biopsy from more than 200 cases with lung cancer(LC),gastric cancer(GC),large intestic cancer(LIC),hepatic cell cancer(HCC),breast cancer(BC),nasopharyngeal cancer (NC),thyroid cancer(TC),cervical cancer(CC),and pleura effusion from 38 cases with LC,peritoneal effusion from 32 cases with HCC were selected.The control group was 45 of blood samples from normal individual.We also investigated p53 gene mutations in patients with mucosal atypical hyperplasia of bronchus and stomach. Results:In the control group,no mutation was found in its blood samples.In the other groups,about 30%～50% cases of LC, GC,LIC,HCC,BC,NC,TC had p53 mutations in their biopsy,the positive mutation rate in pleura effusion or in peritoneal effusion samples was nearly equal to that in biopsy.It also found that the two years′ mortality of patients with p53 mutation arose.Conclusion:p53 gene sequencing is valuable in clinical genetic diagnosis and prognosis.

关 键 词：肿瘤 P53基因 突变 测序 

分 类 号：R730.43[医药卫生—肿瘤]