三氧化二砷对肝癌细胞株SMMC-7721、HepG2抑制作用的研究  

Experiment study on arsenic trioxide and other anti-tumor drugs' effects on human hepatocellular carcinoma cell lines SMMC-7721,HepG2

作　　者：周艳[1] 顾星星[1] 胡亚娥[1] 王东[2] 于志坚[1] 

机构地区：[1]南通医学院生物化学教研室,南通226001 [2]南通医学院附属医院肿瘤中心

出　　处：《南通大学学报（医学版）》2005年第1期10-12,共3页Journal of Nantong University(Medical sciences)

摘　　要：目的 :研究三氧化二砷 (As2 O3)对肝癌细胞株的抑制作用。方法 :用不同浓度的 As2 O3处理人肝癌细胞株 SMMC- 772 1、Hep G2及正常人肝细胞株 L- 0 2 ,应用四甲基偶氮唑盐比色法 (MTT法 )观察 As2 O3对细胞生长的影响 ,并与 3种常用抗癌药羟基喜树碱 (HCPT)、顺铂 (DDP)及 5 -氟脲嘧啶 (5 - Fu)的抑制率进行比较。结果 :低浓度的 As2 O3、HCPT、DDP及 5 - Fu对 SMMC- 772 1、Hep G2两种肝癌细胞株的抑制率分别是 86 .3% ,4 3.2 % ;4 .2 % ,4 2 .1%和 76 .7% ,4 5 .6 % ;19.8% ,18.6 % ,As2 O3的抑瘤作用最强 (P<0 .0 5 ) ,而对正常人肝细胞株的抑制较弱。浓度≥ 2 .0 μm ol/ L 的 As2 O3对 SMMC- 772 1和 Hep G2的抑制作用差异均无显著性 (P>0 .0 5 )。结论 :As2 O3体外试验能有效抑制肝癌细胞株的生长 ,且在一定浓度范围内 。Objective:To study the effects of arsenic trioxide (As 2O 3) on inhibiting the proliferation of hepatocellular carcinoma cell lines.Methods:The human hepatocellar carcinoma cell lines SMMC-7721 and HepG2,ant the human normal liver cell line L-02 were treated with As 2O 3 in various concentration for various days,and then observed and analyzed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, to detect the inhibitory rate of As 2O 3 and other anticancer drugs (HCPT, DDP and 5-Fu).Results:As compared with other 3 kinds of anticancer drugs, the inhibitiion of As 2O 3 was the most obvious(P<0.05). The inhibition of As 2O 3 in the groups with the concentration above 2.0μmol/L were of no difference (P>0.05), but the inhibition was not obvious for L-02 cell.Conclusion:As 2O 3 has obvious antitumor activity on hepatocellular carcinoma cell lines,and the inhibitory rate was dependent on As 2O 3 concetration and treatment time.

关 键 词：原发性肝细胞癌 三氧化二砷 四甲基偶氮唑盐比色法 5-氟脲嘧啶 

分 类 号：R735.7[医药卫生—肿瘤]