PCR扩增Sry基因进行鲸类动物性别的鉴定  

Sex Identification of Cetaceans by PCR with Sry-Specific Primers

作　　者：王加连[1] 杨光[1] 周开亚[1] 魏辅文[2] 严洁[1] 

机构地区：[1]南京师范大学生命科学学院遗传资源研究所,南京210097 [2]中国科学院动物研究所,北京100080

出　　处：《兽类学报》2005年第1期20-23,共4页Acta Theriologica Sinica

基　　金：国家自然科学基金资助项目 ( 3 0 2 70 2 12和 2 0 0 70 116) ;国家自然科学基金杰出青年基金资助项目 ( 3 0 12 5 0 0 6) ;江苏省"青蓝工程"中青年学术带头人科研基金;国家"2 11"工程"十五"建设资助项目

摘　　要：哺乳动物Y染色体短臂上的Sry基因决定雄性发育方向。本研究参照哺乳动物Sry基因保守区序列设计引物 ,以非性别特异性的线粒体DNA细胞色素b基因作为阳性对照 ,用PCR扩增江豚、长喙真海豚等鲸类动物的Sry基因片断并对其进行凝胶电泳分析来鉴定鲸类动物的性别。通过此方法对 87个已知性别鲸类动物标本的检验 ,结果完全正确 ,并进一步应用此方法成功地完成了另外 33个未知性别鲸类标本的性别鉴定。由此建立了一套简单、快速。Sex-determining region Y (Sry) gene located in the short arm of the mammalian Y chromosome directs the sexual development of male. In this study, a 221-bp fragment of cetacean Sry genes was amplified by polymerase chain reaction (PCR) through a pair of primers designed based on the conserved region of other mammalian Sry genes. Eighty seven sex-known cetacean specimens in Nanjing Normal University were accurately determined sex with the simultaneous amplification of mitochondrial DNA cytochrome b (cyt b) gene fragments as positive control, which proved the efficiency of the present molecular technique. The established molecular method was applied to sex eighteen finless porpoises Neophocaena phocaenoides, two bottlenose dolphins Turiops trucatus, three indo-pacific bottlenose dolphins T. aduncus, three baiji Lipotes vexillifer, five striped dolphins Stenella coeruleoalba, and two pantropical spotted dolphin S. attenuata. This study provided a simple, rapid, and accurate approach for cetacean sex identification.

关 键 词：SRY基因 性别鉴定 标本 性发育 PCR扩增 鉴定 特异性 鲸类动物 哺乳动物 引物 

分 类 号：Q959.841[生物学—动物学] S973[农业科学—捕捞与储运]