肝刺激因子对人肝癌细胞EGFR/DNA表达的影响  

Effect of Hepatic Stimulator Substance on the Expression of EGFR/DNA of Human Hepatoma Cells by Means of Bivariated Cellular Immunofluorescence Technique

作　　者：王天翔[1] 金志明[2] 袁建明[2] 朱上林[1] 

机构地区：[1]上海第二医科大学瑞金医院外科,上海200025 [2]上海第二医科大学瑞金医院卢湾分院外科,上海200025

出　　处：《上海第二医科大学学报》2005年第2期141-143,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金 (39470 677)资助项目

摘　　要：目的检测肝刺激因子 (HSS)对人肝癌细胞EGFR/DNA表达的影响。方法双参数流式细胞免疫荧光技术检测肝癌细胞EGFR/DNA的表达。结果HSS下调肝癌细胞DNA表达 ,随时间延长下调程度逐渐增加 ,至 2 4h时DNA表达显著下降(P <0 .0 5 ) ;HSS迅速下调EGFR表达 ,作用 12h即呈现显著差异 (P <0 .0 5 )。Objective To assay the effect of hepatic stimulator substance (HSS) on the expression of EGFR/DNA of human hepatoma cells. Methods Bivariated cellular immunofluorescence technique was used for study. Results HSS decreased the expression of DNA. With the time course, the extent of decrease of DNA was improved. There was a significant difference between the expression of control group and experimental group after 24 h (P< 0.05). HSS decreased the expression of EGFR promptly, and a significant difference was observed after 12 h (P<0.05). Conclusion The expression change of EGFR is probably one of the mechanisms of the effect of HSS on the growth of hepatoma cells.

关 键 词：EGFR DNA表达 人肝癌细胞 肝刺激因子 调肝 流式细胞 肝癌细胞生长 HSS 免疫荧光技术 变化 

分 类 号：R735.7[医药卫生—肿瘤] R512.62[医药卫生—临床医学]