宁南霉素产生菌的选育和发酵条件研究  

STUDIES ON THE SCREENING AND FERMENTATION OF HIGH PRODUCTION STRAIN OF NINGNANMYCIN

作　　者：胡厚芝[1] 陈家任[1] 向固西 徐生林[2] 沈锁彬 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]四川抗菌素工业研究所,成都610051

出　　处：《微生物学报》1993年第6期446-453,共8页Acta Microbiologica Sinica

摘　　要：以诺尔斯链霉菌西昌变种，作为原始菌株，利用紫外线，＾３２Ｐ，镅铍中子源复合因子处理，再经自然分离，获得一株纯化株１６Ａ－６－１４６－７－９８－６－５（简称１６Ａ－６－５）。此突变株在高氏一号琼脂等培养基上，其培养特征和形态特征与原始菌株有明显区别。在玉米粉，花生饼粉培养基中，起始ｐＨ２．７、２８℃，摇瓶振荡培养７２小时，宁南霉素含量为５５００ｕ／ｍｌ，效价比原始菌株提高６倍。The high-yield Ningnanmycin producing mutant No.16A-6-5 of S.noursei var.xichangensis was isolated after treating the parent strain with the complex effects ofUV,^(32)p,and LiCI.The productive activity character of the high yield strain was further stabilized byusing the single spore isolation technique.The optimum fermentation condition was studied and confirmed:the optimumtemperature for Ningnanmycin biosynthesis was 28 C,the ratio of aeration was 1:1.5V/V,the agitation speed was 220 r/min,the titer of Ningnanmyein under theseimproved conditions was around 5 500 u/ml,that showed about 6-fold-higher yield thanthe control.It was shown that the time course of fermotation of the new strain was shorter,temperature was normal,the genetic properties were stable.It was a high productivestrain superior to parent strain.

关 键 词：宁南霉素 链酶菌 发酵 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]