长梗木霉纤维素酶的产生及提取  

STUDIES ON THE CELLULASE PRODUCTION AND EXTRACTION FROM TRICHODERMA LONGIBRACHIATUM

作　　者：谭宏[1] 刘淑欢[1] 李剑英[1] 郭楚盛[1] 

机构地区：[1]广东省微生物研究所,广州510070

出　　处：《微生物学通报》1993年第2期90-93,共4页Microbiology China

摘　　要：对长梗木霉(Trichoderma Longibrachiatum)ANU_3-958纤维素酶的产生及提取进行了研究。结果表明,固体曲培养144小时,固体曲与浸提液比例为1:7(W/V),采用硫酸铵分级沉淀法时所得纤维素酶活力最高。所得冻干纤维素酶粉经测定:羧甲基纤维素(CMC)酶活最高为2788.89IU/g,滤纸糖酶活(FPA)最高为79.44IU/g。相对固体曲得率平均为13.28%。The cellulase production and extraction from Trichoderma Longibrachiatum ANU_3-958 were reported. The results indicated that maximum enzyme activity can be obtained when the fungus was grown on a solid medium for 144 hours, the proportion between the solid culture and the extracting agent was about 1:7(W/V) and extract was precipitated twice by adding solid (NH_4)_2SO_4. Cellulase powder obtained by freeze drying was analysed for its related enzyme activities. The highest CMC and FPA enzyme activity are about 2788.89IU/g enzyme powder and 79.44IU/g enzyme powder respectively. The yield for the solid culture is about 13.28% averagely.

关 键 词：长梗木霉 纤维素酶 

分 类 号：Q939.506[生物学—微生物学]