Effects of intrathecal 6-hydroxydopamine,α_1 and α_2 adrenergic receptor antagonists on antinociception of propofol in mice  

作　　者：Zhi-junGE Yin-mingZENG Yong-feiTAN 

机构地区：[1]JiangsuProvinceKeyLaboratoryofAnesthesiology,XuzhouMedicalCollege,Xuzhou221002 [2]JiangsuProvincePeople'sHospitalofYixing,Yixing214200,China [3]JiangsuProvinceKeyLaboratoryofAnesthesiology,XuzhouMedicalCollege,Xuzhou221002,China//JiangsuProvincePeople'sHospitalofYixing,Yixing214200,China

出　　处：《Acta Pharmacologica Sinica》2005年第2期186-191,共6页中国药理学报（英文版）

摘　　要：Aim:To investigate the relationship between spinal cord norepinephrine,α_1 and α_2 adrenergic receptors and antinociception of propofol in mice.Methods: Kunming mice were used.Antinociceptive tests were investigated with the tail- immersion test and the acetic acid-induced writhing test.The effects of subcuta- neous(sc),intrathecal(ith)and intracerebroventricular(icv)injection propofol on pain threshold were observed.The influences of pretreatment with ith 6- hydroxydopamine,α_1R antagonist prazosin,or α_2R antagonist yohimbine on the antinociception of propofol were studied.Results:Significant antinociception was produced by propofol(25,50 mg/kg,sc)and propofol(20,40μg,ith)in tail- immersion test and acetic the acid-induced writhing test(P<0.05 or P<0.01).Icv propofol(10,20,and 40 μg)did not produce any effect on pain threshold in mice (P>0.05).The 6-hydroxydopamine(5 and 10μg),prazosin(5 and 10μg),or yohimbine(5 and 10μg)ith alone did not affect basal tai-flick latency(TFL)in conscious mice,but significantly reduced the TFL as measured by tail-immersion test in propofol(50mg/kg,sc)-treated mice,compared with basal TFL and ve- hicle groups(P<0.05 or P<0.01).Conclusion:The spinal cord is a target of propofol antinociception.In mice propofol antinociception is partly mediated by spinal norepinephrine,α_1R and α_2R.Aim:To investigate the relationship between spinal cord norepinephrine,α_1 and α_2 adrenergic receptors and antinociception of propofol in mice.Methods: Kunming mice were used.Antinociceptive tests were investigated with the tail- immersion test and the acetic acid-induced writhing test.The effects of subcuta- neous(sc),intrathecal(ith)and intracerebroventricular(icv)injection propofol on pain threshold were observed.The influences of pretreatment with ith 6- hydroxydopamine,α_1R antagonist prazosin,or α_2R antagonist yohimbine on the antinociception of propofol were studied.Results:Significant antinociception was produced by propofol(25,50 mg/kg,sc)and propofol(20,40μg,ith)in tail- immersion test and acetic the acid-induced writhing test(P<0.05 or P<0.01).Icv propofol(10,20,and 40 μg)did not produce any effect on pain threshold in mice (P>0.05).The 6-hydroxydopamine(5 and 10μg),prazosin(5 and 10μg),or yohimbine(5 and 10μg)ith alone did not affect basal tai-flick latency(TFL)in conscious mice,but significantly reduced the TFL as measured by tail-immersion test in propofol(50mg/kg,sc)-treated mice,compared with basal TFL and ve- hicle groups(P<0.05 or P<0.01).Conclusion:The spinal cord is a target of propofol antinociception.In mice propofol antinociception is partly mediated by spinal norepinephrine,α_1R and α_2R.

关 键 词：PROPOFOL spinal cord ANTINOCICEPTION prazosin yohimbine norepinephrine 

分 类 号：R971[医药卫生—药品]