查尔酮对人胃癌BGC823细胞生物学行为影响的初步研究  

Elementary study of the Effect of Chalcone on Cellular Biological Behavior of Human Stomach Cancer BGC823

作　　者：王丽[1] 阎蕾[2] 张景怡[2] 冯正明[2] 贾光[2] 张宝旭[2] 

机构地区：[1]内蒙古科技大学公共卫生学院,内蒙古包头014010 [2]北京大学医学部

出　　处：《包头医学院学报》2004年第4期265-267,共3页Journal of Baotou Medical College

基　　金：211工程肿瘤学科群课题太极抗癌基金资助

摘　　要：目的:研究查尔酮对人胃癌BGC823细胞生长抑制、细胞周期阻滞及诱导细胞凋亡的作用,为寻找预防肿瘤药物提供一定的线索。方法:细胞生长抑制采用MTT比色法检测,细胞周期阻滞用流式细胞仪检测,细胞凋亡用流式细胞仪及琼脂糖凝胶电泳检测。结果:浓度为6.25、12.5、25和50μmol／L的查尔酮呈剂量依赖性抑制BGC823细胞增殖。查尔酮诱导细胞凋亡率是对照的2.71、2.79、4.74倍。琼脂糖凝胶电泳可观测到12.5／μmol／L、25μmol／L有DNA"梯子"现象。结论:查尔酮可以抑制BGC823细胞生长,并呈剂量-反应关系。查尔酮有将BGC823细胞增殖周期阻滞在G0/G1期趋势,查尔酮可以抑制肿瘤细胞的生长和增殖。Objective:To understand how chalcone inhibits the growth of human stomach cancer BGC823 ,arrests the cell cycle progress and induces cell apoptosis in order to explore a new clue to finding a cure for stomach cancer. Methods: Cytotoxicity was determinded by MTT chromatometry, cell cycle progress and apoptosis were determined by flow cytometry. DNA ladder was examined by Agarose Gel Electrophoresis. Results: Cytotoxicity induced by chalcone were increased in a dose - dependent manner ( r >0.91, P <0.01).6.25umol/L chalcone can arrest the cell cycle at the G0/G1 phase( P <0.05). Agarose Gel Electrophoresis showed that the phenomena of DNA 'ladder' appeared in the chalcone group at the doses of 12. 5 ,25umol/L. Conclusion : chalcone can inhibit the growth of BGC823 and arrest the cell cycle.

关 键 词：查尔酮 人胃癌BGC823细胞 细胞增殖抑制 细胞周期阻滞 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]