ENU诱变获得3种眼突变小鼠模型的研究  

Primary Report of Three Mouse Models Showing Eye Mutation Induced by ENU

作　　者：薛整风 茅慧华 赵明[2] 邵义祥 陈兵 吴宝金[2] 李厚达 

机构地区：[1]扬州大学比较医学中心,江苏扬州225009 [2]扬州大学医学院,江苏扬州225001

出　　处：《扬州大学学报（农业与生命科学版）》2004年第4期68-71,共4页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家"十五"科技攻关项目(2001BA710B);江苏省高校自然科学基金资助项目(03KJB180155)

摘　　要：选择8～10周龄雄性C57BL/6J小鼠120只,腹腔注射乙烷基亚硝基脲(ENU)100mg.kg-1,每周1次,连续3周。将处理雄鼠与同品系母鼠交配,在其后代小鼠中筛选出眼部突变个体,并对突变的个体进行遗传试验,以建立人类遗传性眼病小鼠模型。结果表明:在筛查的3500只诱变小鼠中得到85只眼部异常的突变体,其中能遗传眼部异常表型的突变种3例,1例为双角膜浑浊,其后代表型复杂,2/3为虹膜缺损,1/3为角膜浑浊;1例为小睑裂伴角膜变性浑浊;1例为角膜变性浑浊。这3种突变表型都表现为显性遗传,且均为人类先天性眼病常见类型,说明诱变可以获得人类遗传性眼病的小鼠模型。To obtain eye mutation mice induced by ENU which would offer the models for human eye diseases, 120 C57BL/6J male mice about 8-10 weeks old were injected intraperitoneally with a dose of ENU 100 mg·kg^(-1) once a week and total three times, and mated with the same strain female mice. Their progenies were screened for visible eye mutation, and the mutant mice were mated with the same strain mice to confirm whether their mutation phenotypes were of heredity. 3()500 progenies mice had been screened and 85 mice with abnormal phenotypes in eyes were found, three of them showing dominant heredity. They were of C57BL/6J-Y24, C57BL/6J-Y343 and C57BL/6J-Y2860. C57BL/6J-Y24 was nubecula in two eyes. But the phenotypes of C57BL/6J-Y24′s progenies were very complicated: 2/3 of them showing coloboma of iris and 1/3 of them being of nubecula. C57BL/6J-Y343′s palpebral fissure was small and went with nubecula. And C57BL/6J-Y2860 was of nubecula. The phenotypes of these three mutants were similar to the common eyes transmissibility diseases of human beings. We could gain the mice models of human′s eyes transmissibility diseases by ENU inducing.

关 键 词：小鼠 乙烷基亚硝基脲 突变 眼 模型 

分 类 号：R394.8[医药卫生—医学遗传学] S858.91[医药卫生—基础医学]