非小细胞肺癌中转化生长因子βⅠ型受体基因的缺陷表达(英文)  

Defective expression of TGFβR Ⅰ gene in non-small cell lung cancer

作　　者：张洪涛[1] 赵军[2] 陈晓峰[3] 曾福礼[4] 

机构地区：[1]苏州大学生命科学学院医学遗传学研究室,江苏苏州215006 [2]苏州大学附属第一医院外科,江苏苏州215006 [3]上海市肺科医院外科,上海200433 [4]兰州大学生命科学学院,甘肃兰州730000

出　　处：《兰州大学学报（自然科学版）》2005年第1期45-51,共7页Journal of Lanzhou University(Natural Sciences)

基　　金：SupportedbyGrantsfromChina'sNationalNaturalScienceFoundation(30400533)ScienceandTechnologyCommitteeofJiangsuProvince(BK2004402)

摘　　要：转化生长因子β(TGF-β)信号转导通路的紊乱可使细胞逃避TGF-β介导的生长抑制效应,从而导致多种肿瘤的发生.本研究探讨了转化生长因子βⅠ型受体(TGFβRⅠ)在非小细胞肺癌(NSCLC)发生中的作用.采用免疫组化进行TGFβRⅠ基因的表达分析:采用单链构象多态性(SSCP)分析TGFβRⅠ基因结构的变异.结果发现37.2％NSCLC中TGFβRⅠ的表达下降,表明TGFβRⅠ在NSCLC发生中起着重要的作用.同时在TGFβRⅠ基因的第7号内含子中发现了一个单核苷酸多态性(SNP),SNP与TGFβRⅠ的表达下降无显著关系(P>0.05).Many malignant tumor cells, including non-small cell lung cancer (NSCLC) cells, are frequently resistant to transforming growth factor β (TGF-β)-mediated signal transduction. The TGF-β signal is transduced through a pair of transmembrane serine-threonine kinase receptors named receptor type I and Ⅱ (TGFβR Ⅰand TGFβR Ⅱ). Reduced expression of TGFβR Ⅰ was found in various types of human carcinomas. To investigate whether the TGFβR Ⅰ gene plays a role as a tumor suppressor in the pathogen-esis of NSCLC, we performed the immunohistochemical and molecule structural analyses of TGFβR Ⅰ in tumor and the paired normal tissues from 43 resection specimens. TGFβR Ⅰ expression was studied by immunohistochemistry, and the TGFβR Ⅰ gene was examined by polymerase chain reaction-single strand conformation polymorphism analysis (PCR-SSCP). The results obtained in this study demonstrated loss or reduction of TGFβR Ⅰ expression in 16 (37.2%) of the 43 tumor tissues. However, no somatic mutation other than a polymorphism was identified. The present study suggested that reduced TGFβR Ⅰ expression could contribute to the development of malignant phenotype of NSCLC, even though the genetic mutation of the TGFβR Ⅰ gene is not involved in the tumorigenesis of NSCLC and does not appear to be directly responsible for reduced expression of TGFβR Ⅰ.

关 键 词：非小细胞肺癌 转化生长因子βⅠ型受体 免疫组化 基因突变 多态性 

分 类 号：R734.2[医药卫生—肿瘤] R394[医药卫生—临床医学]