Metabolic Engineering of Tropane Alkaloid Biosynthesis in Plants  

作　　者：LeiZHANG Guo-YinKAI Bei-BeiLU Han-MingZHANG Ke-XuanTANG Ji-HongJIANG Wan-ShengCHEN 

机构地区：[1]SchoolofPharmacy,TheSecondMilitaryMedicalUniversity,Shanghai200433,China／／KeyLaboratoryforBiotechnologyonMedicinalPlantofJiangsuProvince,XuzhouNormalUniversity,Xuzhou221116,China [4]PlantBiotechnologyResearchCenter,SchoolofAgricultureandBiology,Fudan-ShanghaiJiaotongUniversity-NottinghamPlantBiotechnologyResearchandDevelopmentCenter,SchoolofLifeSciencesandTechnology,ShanghaiJiaotongUniversity [5]SchoolofPharmacy,TheSecondMilitaryMedicalUniversity,Shanghai200433,China [6]KeyLaboratoryforBiotechnologyonMedicinalPlantofJiangsuProvince,XuzhouNormalUniversity,Xuzhou221116,China

出　　处：《Journal of Integrative Plant Biology》2005年第2期136-143,共8页植物学报（英文版）

摘　　要：Abstract: Over the past decade, the evolving commercial importance of so-called plant secondary metabolites has resulted in a great interest in secondary metabolism and, particularly, in the possibilities to enhance the yield of fine metabolites by means of genetic engineering. Plant alkaloids, which constitute one of the largest groups of natural products, provide many pharmacologically active compounds. Several genes in the tropane alkaloids biosynthesis pathways have been cloned, making the metabolic engineering of these alkaloids possible. The content of the target chemical scopolamine could be significantly increased by various approaches, such as introducing genes encoding the key biosynthetic enzymes or genes encoding regulatory proteins to overcome the specific rate-limiting steps. In addition, antisense genes have been used to block competitive pathways. These investigations have opened up new, promising perspectives for increased production in plants or plant cell culture. Recent achievements have been made in the metabolic engineering of plant tropane alkaloids and some new powerful strategies are reviewed in the present paper.Abstract: Over the past decade, the evolving commercial importance of so-called plant secondary metabolites has resulted in a great interest in secondary metabolism and, particularly, in the possibilities to enhance the yield of fine metabolites by means of genetic engineering. Plant alkaloids, which constitute one of the largest groups of natural products, provide many pharmacologically active compounds. Several genes in the tropane alkaloids biosynthesis pathways have been cloned, making the metabolic engineering of these alkaloids possible. The content of the target chemical scopolamine could be significantly increased by various approaches, such as introducing genes encoding the key biosynthetic enzymes or genes encoding regulatory proteins to overcome the specific rate-limiting steps. In addition, antisense genes have been used to block competitive pathways. These investigations have opened up new, promising perspectives for increased production in plants or plant cell culture. Recent achievements have been made in the metabolic engineering of plant tropane alkaloids and some new powerful strategies are reviewed in the present paper.

关 键 词：biosynthesis pathway genetic transformation HYOSCYAMINE plant secondary metabolic engineering SCOPOLAMINE tropane alkaloids 

分 类 号：Q946.8[生物学—植物学] Q943.2