羊肚菌多糖提取、分离条件的选择  

Study on Extraction and Separation of Polysaccharide of Morchella esculenta Cultured by Fermentation

作　　者：武秋立[1] 安家彦[2] 

机构地区：[1]天津大学化工学院,天津300072 [2]大连轻工业学院生物与食品工程学院,辽宁大连116034

出　　处：《食品科学》2005年第1期116-120,共5页Food Science

摘　　要：为了提高羊肚菌液体深层培养的多糖得率,本文研究了影响羊肚菌胞内、胞外多糖得率的各种因素。经试验确定羊肚菌胞外多糖分离优化条件为:沉淀温度-15℃,沉淀乙醇倍数3倍,沉淀时间24h,沉淀pH值为7。羊肚菌胞内多糖的浸提优化条件为:采用热水浸提,浸提温度85℃,浸提时间2h,浸提加水比50:1,浸提pH值为7,浸提次数两次。对比试验说明,优化效果显著。经SephadexG-150柱层析结果表明,羊肚菌胞外多糖由5种大分子多糖组成,而胞内多糖仅由2种组成。For improving the polysaccharide yield, the factors influencing the extraction and the separation of polysaccharide of Morchella esculenta cultured by submerged fermentation were studied. The optimum conditions of alcohol-precipitating exopolysaccharide were: 3 times alcohol; freezing time 24 hours; temperature -15℃ and pH 7. The optimum extracting conditions of endo-polysaccharides from mycelia were given below: adding 50 times volume water to dry mycelia; extracting time 120 min; temperature 85℃, pH 7 and extracting two times. The study proved that there were five kinds of components in exo- polysaccharides of Morchella esculenta and two kinds in endo-polysaccharides by Sephadex-150 column chromatograph.

关 键 词：羊肚菌 胞外多糖 胞内多糖 提取 

分 类 号：S567.3[农业科学—中草药栽培] Q539[农业科学—作物学]