毛茛甙的抗诱变作用及代谢转化  

ANTIMUTAGENIC ACTIVITY AND METABOLIC TRANSFORMATION OF RANUNCULIN BY RAT LIVER MICROSOMES

作　　者：李润沼[1] 裴惠平[2] 籍秀娟[1] 

机构地区：[1]中国医学科学院药物研究所 [2]河南新乡市中心医院,新乡453000

出　　处：《药学学报》1993年第7期481-485,共5页Acta Pharmaceutica Sinica

摘　　要：研究表明,毛茛甙可显著抑制MMC和MMS等诱变剂对沙门氏菌TA_(100)和TA_(102)回复突变作用;对MMC诱发的小鼠PEC徽核抑制率达56.5%。代谢研究表明:毛茛甙抑制DNA生物合成的作用可被肝微粒体酶和胞浆液分别降低46和12%;RP-HPLC测定显示,体外毛茛甙可被肝微粒代谢转化。Ranunculin (RAN) was shown to be an antimutagenic agent selectively against mitomycin C (MMC) or methyl methane sulfanate (MMS) treated Salmonella typhimminum TA_(100)/TA_(102). It decreased the formation of micronucleus of MMC induced polychromatic erythrocytes (PEC) from 46±9.2% to 20±6% in mice. The inhibition of RAN on the incorporation of ~3H-TdR into DNA disappeared after incubation with rat liver microsomes and cytoplasm since RAN was found to be metablized by rat liver microsomes in vitro, resulting in a new absorbance peak at 258 nm, determined by RP-HPLC. The data show that RAN may have both antimutagen and antitumor activity, but the latter action may disappear by metabolic transformation.

关 键 词：毛茛甙 抗诱变作用 代谢转化 

分 类 号：R965.2[医药卫生—药理学]