水稻脂质氧化酶同工酶种质储藏特性的研究  

Study on the Difference of the Durable Storage Character of Rice Grain with the Loss of Lipoxygenase Isoenzymes

作　　者：宋美[1,2] 张瑛 吴敬德[3] 余增亮[1] 童继平[3] 郑乐娅[2] 程灿[4] 刘斌美[4,5] 佘德红[3] 吴跃进[1] 

机构地区：[1]中国科学院离子束生物工程重点实验室 [2]安徽农业大学生命科学学院,合肥230036 [3]安徽省农业科学院水稻研究所 [4]安徽农业大学生命科学学院 [5]安徽省农业科学院水稻研究所,合肥230031

出　　处：《中国农学通报》2005年第2期65-68,共4页Chinese Agricultural Science Bulletin

基　　金：科技部"863"项目(2001AA241018);中国科学院创新项目;安徽省科技厅重大科技专项(01603002)。

摘　　要：对水稻脂质氧化酶(LOX)同工酶缺失的单体及聚合体人工老化实验研究表明:不同LOX的缺失突变体之间,以LOX-1,LOX-2、LOX-3全缺的D1308、D1311的发芽率始终最高,老化指数最低;LOX-1缺失所造成的影响要大于LOX-2缺失和LOX-3缺失。LOX缺失的单体及聚合体具有较好的储藏特性,LOX的缺失对保持种子生活力和延缓稻谷陈化变质具有重要的作用。进一步的分析认为LOX-1的缺失阻断或延缓了LOX-2和LOX-3所启动的脂质过氧化反应,从而延缓了稻谷的陈化变质,减轻种子生活力的下降。LOX-1可能是控制稻谷陈化变质和种子衰老的关键基因。It is shown through accelerated aging tests that among all materials D1308, D1311 with null- LOX-1, LOX-2 and LOX-3 have the highest germination ratio and the lowest aging index ;wanjian2090 and D1305 with null- LOX-1?LOX-2 are more storageble than those with null- LOX-2, LOX-3 and those with null- LOX-2 or null- LOX-3; D1304, D1312 with null- LOX-2, LOX-3 are not apparently more storageble than those with null- LOX-2 or null- LOX-3.It indicates the effects of null- LOX-1 are more profound than those of null- LOX-2 or null- LOX-3. The materials lacking single or compound lipoxygenase have much better storage character than those common ones .The lacking of lipoxygenase can stave the rice from deteriation effectively and the lacking of LOX-1 cut off or stave the lipid superoxidation which are triggered by LOX-2 and LOX-3.Above all LOX-1 is probably the key gene responsible for the long life span of rice grains.

关 键 词：LOX 水稻 种子生活力 储藏 种质 稻谷 同工酶 延缓 脂质氧化 关键基因 

分 类 号：S511[农业科学—作物学] S572