大连湾牡蛎蛋白的分离提取及分子量测定  

The Extraction and Separation of the Protein from Ostrea Talienwhanensis Crosse and the Determination of the Molecular Weight

作　　者：崔韶晖[1] 王艳颖[1] 崔杰[1] 王岩[1] 左明[1] 宁洪良[1] 

机构地区：[1]大连民族学院生物工程系,辽宁大连116600

出　　处：《大连民族学院学报》2005年第1期53-54,73,共3页Journal of Dalian Nationalities University

基　　金：大连民族学院博士科研启动基金资助项目(20036209);大连民族学院太阳鸟计划(030507)资助项目.

摘　　要：以大连湾牡蛎为实验材料.首先采用冰醋酸粗提法提取牡蛎蛋白,然后用葡聚糖凝胶(Sephadex-G100)进行柱层析分离,SDS—聚丙烯酰胺凝胶垂直板电泳分离蛋白,Kodak凝胶成像分析仪分析及测定分子量.结果显示大连湾牡蛎中含有分子量为55kd的牡蛎特征蛋白,同时分离出其它4种蛋白质,分子量介于33.0~63.4kd之间.Ostrea talienwhanensis crosse in Dalian Bay was taken as the experiment material. Protein was first extracted with acetic acid and was purified by Sephadex G-100 gel filtration chromatography, then the protein was separated by SDS-PAGE electrophoresis, and the molecular weight of the protein with Gel Image Station (Kodak) was determined. The results showed that oyster contained the protein of characterized of the oyster talienwhanenisis crosse 55kd and others 4 specie proteins whose molecular weight is between 33.0kd^63.4kd in ostrea talienwhanensis crosse.

关 键 词：牡蛎 蛋白质 Sephadex—G100柱层析 SDS—PAGE电泳 

分 类 号：Q51[生物学—生物化学]