金耳的液体发酵研究  

A Study on the Submerged Culture Conditions of Tremella aurantialba

作　　者：汪虹[1] 瞿伟菁[2] 曹群华[2] 

机构地区：[1]上海市农业遗传育种重点实验室上海市农业科学院食用菌研究所,上海201106 [2]华东师范大学生命科学院,上海200062

出　　处：《食用菌学报》2003年第4期29-33,共5页Acta Edulis Fungi

基　　金：上海市高等学校科研基金资助项目(JG9507)

摘　　要：研究表明,金耳(Tremellaaurantialba)液体发酵最适培养基组成为玉米粉2%,蔗糖3%,酵母膏0.1%,KH2PO40.1%,MgSO40.05%;培养基最佳初始pH、250mL三角瓶装量、接种量、种子菌龄分别是3.5、80mL、10%和120h;用10L发酵罐发酵时,最佳培养条件是种子种龄144h、28℃、接种量10%、通风量2.5L min。The results of orthogonal test showed that the optimum medium for the mycelial growth of Tremella aurantiabla was composed with 2% corn powder, 3% sucrose, 0.1% yeast extract, 0.1% KH_2PO_4 and 0.05% MgSO_4,and that the optimum initial pH, medium capacity of 250mL Erlenmeyer flask, inoculation rate and spawn age were 3.5, 80mL, 10% and 120h resp.. When the mycelia of Tremella aurantiabla were cultured in 10L fermentor, the optimum spawn age, culture temperature, inoculation rate and ventilation rate for the mycelial growth of Tremella aurantiabla were 144h, 28℃, 10% and 2.5L/min resp..

关 键 词：金耳 液体发酵 培养基 培养条件 正交试验 菌丝产量 

分 类 号：S567.340.35[农业科学—中草药栽培]