不同培养基脆性X表达率研究  

Srudy of fragile X chromosome expression with different culture media

作　　者：陈争[1] 张东晓[1] 黄春浓[1] 陆雪芬[1] 

机构地区：[1]中山医科大学医学遗传教研室,蚌埠医学院生物教研室,广州医学院神经科学研究所

出　　处：《中国优生与遗传杂志》1994年第F08期29-31,共3页Chinese Journal of Birth Health & Heredity

摘　　要：采用ＴＣ１９９和ＲＰＭＩ１６４０培养基，加入ＦｕｄＲ和咖啡因诱导剂，分别对１４例ｆｒａ（Ｘ）阳性个体和６例Ｆｒａ（Ｘ）阴性个体进行脆性Ｘ染色体表达率对比研究发现：两种方法均能检出Ｆｒａ（Ｘ）染色体，其表达率无明显差异（０．２＜Ｐ＜０．５）；而用ＲＰＭＩ１６４０培养制作的标本明显优于ＴＣ１９９培养制作的标本，前者计数１００个中期分裂相所需玻片数为３．４张，而后者需１１．１张，两者比较有显著意义（Ｐ＜０．００１）。By using two different culture media (TC 199 and RPMI 1640),the authors examined 14 persons with fragile X positive and six fragile X negative persons.FudR and caffeine as inducing agents were included in both. Fragile X couled be detected in either of them. The expression rate of fragile X with the two methods had no significant difference(0.2<P<0.5),but the RPMI 1640 medium was superior in specimen quality. In average,only 3.4 slides were required for analyzing 100 division cells by using RPMI 1640 medium as compared to 11.4 slides in the other The difference was highly significant(P<0.001).The result shows that RPMI 1640 medium may replace TC199 for testing fragile X chromosome.

关 键 词：TC1 表达率 检出 阴性 阳性 中期 X染色体 玻片 染色体检测 培养基 

分 类 号：R392.11[医药卫生—免疫学]