人胃粘膜幽门螺杆菌的flaA基因的变异  

Variability of flaA Gene of Helicobacter Pylori from Human Gastric Mucosa

作　　者：余毅 徐智民[2] 欧锡祺 宋姗[2] 张妙英 

机构地区：[1]广东省顺德市大良医院,528300 [2]广州第一军医大学南方医院全军消化内科研究所

出　　处：《现代消化病及内镜杂志》1998年第1期17-20,共4页

摘　　要：我们对52例胃粘膜活检标本和18株 Hp 临床分离株进行 Hp 特异的16S rRNA 基因和鞭毛素 A 基因 PCR 扩增和 PCR-RFLP 及 PCR-RFLP-SSCP 分析,以检测 Hp 鞭毛素 A 基因的变异性,进而研究其与 Hp 致病性的关系。结果对43例 Hp(+)的 flaA 基因 PCR-RFLP(HindⅢ)大约可分7个型(变异检出率为16.3%),而PCR-RFLP-SSCP 可分37个型(变异检出率为86.0%),两者有非常显著性差异。本研究表明,flaA 基因变异性极大,PCR-RFLP-SSCP 是检测其变异的有效方法。To detect the variability and its pathogenecity of Helicobacter pylori(H.pylori),fifty- two biopsy specimens and 18 strains of H.pylori were performed PCR for 16S rRNA gene and flagellin A gene(flaA).PCR-RFLP and PCR-RFLP-SSCP were also performed.RESULTS:Seven flaA genotypes were found by PCR-RFLP in 43 strains of H.pylori(detecting rate of variance was 16.3%),but 37 flaA genotypes were found by PCR-RFLP-SSCP in the same cases(detecting rate of variance was 86.0%),there is very difference between them.CONCLUSION:the study shows that very large variability in flaA gene of H pylori,which were detected by PCR-RFLP-SSCP,an effective method to identify H.pylori,strains in human stomach.

关 键 词：胃粘膜 幽门螺杆菌 flaA基因 基因变异 16rRNA基因 flaA基因变异 

分 类 号：R573[医药卫生—消化系统]