鉴定日本血吸虫cDNA阳性克隆三种方法的比较  

Comparison of Three Methods of Identifying Schistosoma Japonicum Positive cDNA Clones

作　　者：沈定文[1] 李云[1] 詹希美[2] 刘启文[2] 

机构地区：[1]咸宁医学院寄生虫学教研室 [2]中山医科大学寄生虫学教研室

出　　处：《咸宁医学院学报》1996年第2期58-59,73,共3页Journal of Xianning Medical College

基　　金：美国中华医学基金会(CMB)资助课题

摘　　要：本文利用融源表达、平板裂解法和PCR扩增对日本血吸虫cDNA阳性克隆进行鉴定。所有8个阳性克隆均能在宿主菌大肠杆菌Y1089中表达,PCR能扩增出特异性条带,cDNA阳性克隆经限制性核酸内切酶酶解、琼脂糖凝胶电泳后,显示为700-900bp。结果表明,我们所采用的三种方法均能有效地鉴定日本血吸虫阳性克隆。Schistosoma japonicum positive cDNA clones were identified by lysogenic expression, flat lytic method and PCR amplification. All eight positive clones could be expressed in E.coli Y1089 and specifically amplified by PCR. The positive cDNA clones were digested separately by restriction endonuclease EcoRI, followed by agarose gel electrophoresis.The size of them was 700 - 900bp. The results indicated that Schistosoma japonicum positive cDNA clones could be identified effectively by means,of these three methods.

关 键 词：日本血吸虫 DNA 表达 鉴定 大脑 阳性克隆 PCR扩增 限制性核酸内切酶 琼脂糖凝胶电泳 宿主 

分 类 号：R383.24[医药卫生—医学寄生虫学] Q785[医药卫生—基础医学]