逆转录聚合酶链反应检测乙型脑炎病毒核酸  

Detection of Japanese Encephalitis Viral RNA by Reverse Transcription-Polymerase Chain Reaction

作　　者：李刚[1] 王飞[1] 郭日波[1] 柯伟民[1] 罗慧容 

机构地区：[1]中山医科大学传染病学教研室 [2]广州市卫生防疫站

出　　处：《中国人兽共患病杂志》1993年第6期11-12,共2页Chinese Journal of Zoonoses

摘　　要：本文应用逆转录聚合酶链反应(RT-PCR)方法检测乙型脑炎病毒基因。所设计引物在E基因组,引物1位于碱基序列的第1953～1972位,引物2位于第1788—1806位。反应产物为185bp,内含HaeⅢ限制性内切酶位点。产物在含溴化乙锭的2％琼脂糖中电泳。本法可特异性地检测乙型脑炎病毒核酸,并可检出少至5TCID_(50)的病毒RNA。Reverse transcription--polymerase chain reaction (RT-PCR) was developed for the detection of Japanese encephalitis virus gene. Two primers bracketed a 186-nucleotide base in the E gene, corresponding to bases 1788-1972 in the Japanese encephalitis virus genomic RNA sequence. The amplified product including Hae Ⅲ site was shown in the stained 2% agrose gel by ethidium bromide. This method can specifically detect Japanese encephalitis viral RNA from at least 5TCID50 virus.

关 键 词：乙型脑炎病毒 逆转录 聚合酶链反应 

分 类 号：R737.31[医药卫生—肿瘤]