谷氨酸氧化酶电极的研究  

Study of the Enzyme Electrode Based on Glutamate Oxidase

作　　者：杨庆玲[1] 毕可万[1] 

机构地区：[1]中国科学院大连化学物理研究所

出　　处：《生物工程学报》1993年第3期277-281,共5页Chinese Journal of Biotechnology

摘　　要：用戊二醛作交联剂将谷氨酸氧化酶和牛血清白蛋白共交联,置于内层醋酸纤维素抗干扰膜和外层聚碳酸酯扩散控制膜之间,制成酶膜,将其与过氧化氢探头复合制成了扩散控制型谷氨酸氧化酶电极,并构建了采用该电极的流动注射分析系统。酶电极线性范围0—1000mg/L。50s响应电流达稳态值的95%。流动注射分析系统响应时间20s,检测速度60次/h,线性范围5—8000mg/L,酶膜使用寿命两周以上。系统选择性好,仅对干扰物L-谷氨酰胺和L-天冬氨酸有微弱响应。对同一样品连续测定41次的变异系数2.8%。测量味精发酵液和瓦勃呼吸计的结果相吻合。可望应用于味精发酵及食品工业中。A diffusion-controlled enzyme electrode was constructed for L-gluta-mate determination. The glutamate oxidase was immobilized between the cellulose acetate membrane and polycarbonate membrane using serum albumine and glutaraldehyde. The enzyme membrane was attached on the hydrogen peroxide probe surface moistened with electrolyte. The linear range of the enzyme electrode extends up to 1000mg/L. A FLA system using the enzyme electrode was developed. The system exhibited good linearity (5—8000mg/L with r=0.9998), rapid response time (less than 20s) and good selectivity. The electrode can be used continually for more than 2 weeks. The coefficient of variation (CV) of 41 times measurments at the glutamate concentration of 4000mg/L is 2.8%. So its application to fermentation process control and food analysis is very promising.

关 键 词：味精 L-谷氨酸 谷氨酸氧化酶 酶电极 

分 类 号：TS264.23[轻工技术与工程—发酵工程]